Chromatography Resin Information Library
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Request a sample of one of our resins
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Bio-Rad manufactures a wide range of chromatography resins for process separations, providing process developers with great flexibility and high productivity. Our leading chromatography resins for biomolecule purifications include best-in-class ion exchange resins and our innovative mixed-mode resins.
- Nuvia™ Ion Exchange Resins provide ultra–high binding capacity and exceptional resolution across a range of pH and flow rates — ideal for biosimilar and biotherapeutic downstream processes.
- UNOsphere™ Ion Exchange Resins are highly macroporous beads allowing fast linear velocities at low backpressure — designed for high-efficiency capture of biomolecules from crude feedstreams.
- CHT™ and Nuvia™ cPrime™ Mixed-Mode Resins offer unique separation properties and unparalleled selectivity and resolution for a variety of molecules. Combining ion exchange and metal affinity or hydrophobic interactions that enable the separation of biomolecules that appear homogeneous using other methods. These resins can be used at any stage in a purification process.
Ion Exchange Resins: Macro-Prep®, UNOsphere™, and Nuvia™ Matrices
Bio-Rad's ion exchange resins are available in various particle sizes, ionic forms, and purity ranges to offer a variety of purification solutions for process applications. These resins are ideal for biotherapeutic purifications where high productivity, economical use, and process flexibility are critical parameters for success.
| Number | Description | Download |
|---|---|---|
| 6129 | Nuvia™ Q Anion Exchange Resin | Click to download |
| 5987 | Nuvia™ S Cation Exchange Resin | Click to download |
| 6448 | Nuvia™ HR-S High-Resolution Cation Exchange Resin | Click to download |
| 2724 | UNOsphere™ Q Anion Exchange Resin | Click to download |
| 2729 | UNOsphere™ Q Anion Exchange Resin Technical Data | Click to download |
| 2669 | UNOsphere™ S Cation Exchange Resin | Click to download |
| 2678 | UNOsphere™ S Cation Exchange Resin Technical Data | Click to download |
| 5515 | Guidelines for Using UNOsphere™ S Cation Exchange Resin | Click to download |
| 5644 | Macro-Prep® High Q Anion Exchange Resin | Click to download |
| 5643 | Macro-Prep® High S Cation Exchange Resin | Click to download |
| Number | Description | Download |
|---|---|---|
| RP0048 | How to Choose an Industrial Cation Exchanger for IgG Purification | Click to download |
| RP0049 | The Effect of Conductivity on Dynamic Binding Capacity on CEX Resins Tech Poster | Click to download |
| 6439 | Improving Aggregate Removal from a Monoclonal Antibody Feedstream Using High-Resolution Cation Exchange Chromatography | Click to download |
| 6128 | Workflow for Protein Purification from Whey Using Nuvia™ Ion Exchange Media | Click to download |
| 5984 | Nuvia™ S Media: A High-Capacity Cation Exchanger for Process Purification of Monoclonal Antibodies | Click to download |
| 1942 | Macro-Prep® DEAE Support for Chromatography of Biomolecules | Click to download |
| 2774 | Purification of Transgenic Antibody from Corn Seed Using UNOsphere™ S and CHT™ Ceramic Hydroxyapatite Resins | Click to download |
| 2524 | Purification of Horse IgG Using Macro-Prep® DEAE and CHT™ Ceramic Hydroxyapatite Type I Resins | Click to download |
| 1985 | Clearance of Murine Leukemia Virus from a Chimeric Monoclonal Antibody Using Ion Exchange Chromatography | Click to download |
| 1913 | Developing and Scaling Up a Purification Procedure With Two New Ion Exchange Resins (Poster) | Click to download |
| 2204 | Effective Removal of Negatively Charged Interfering Molecules From Proteins | Click to download |
| 1917 | Purification of Human Monoclonal IgM Antibody from Bioreactor Supernatant Using a Combination of Cation and Anion Exchange Chromatography | Click to download |
| 6711 | Purification of Amyloidogenic Transthyretin Protein Using Nuvia™ Q Anion Exchange Resin | Click to download |
| 6720 | Development of a cGMP-Ready Purification Process for Adenovirus Purification (Poster) | Click to download |
| Number | Description | Download |
|---|---|---|
| 10033316 | Instruction Manual, Nuvia™ HR-S Strong Cation Exchange Resins | Click to download |
| 10018215 | Instruction Manual, Nuvia™ S and Q High-Capacity Ion Exchange Resins | Click to download |
| 4110109 | Instruction Manual, UNOsphere™ Q and S Ion Exchange Resins | Click to download |
| LIT271 | Instruction Manual, Macro-Prep® Ion Exchange Resins | Click to download |
Mixed-Mode Resins: Ceramic Apatite and Nuvia™ Matrices
Bio-Rad's mixed-mode chromatography resins offer unique separation properties and unparalleled selectivity and resolution for a variety of molecules. Their unique selectivities allow for purification of complex feeds and the separation of closely related species simply by manipulating a few conditions in one chromatography step. These resins can be used at any stage of purification, from capture to polishing.
| Number | Description | Download |
|---|---|---|
| 6086 | Ceramic Hydroxyapatite Application Guide for Process Development and Scale-Up | Click to download |
| 6242 | Nuvia™ cPrime™ Hydrophobic Cation Exchange Resin | Click to download |
| 5667 | CHT™ Ceramic Hydroxyapatite | Click to download |
| 2156 | CHT™ Ceramic Hydroxyapatite – A New Dimension in the Chromatography of Biological Molecules | Click to download |
| 6432 | MPC™ Ceramic Hydroxyfluoroapatite Resin | Click to download |
| 3111 | CFT™ Ceramic Fluoroapatite Resin | Click to download |
| Number | Description | Download |
|---|---|---|
| 6086 | CHT™ Ceramic Hydroxyapatite Resource Guide – available online at www.bio-rad.com/chtguide | Click to download |
| RP0033 | A Ceramic Hydroxyapatite-Based Purification Platform | Click to download |
| 6549 | Mammalian Virus Purification Using Ceramic Hydroxyapatite | Click to download |
| 6242 | Purification of Recombinant Proteins on Nuvia™ cPrime™ Hydrophobic Cation Exchange Resin: A Simple Approach to Method Development | Click to download |
| 6278 | High-Throughput Calcium Analysis of Hydroxyapatite Column Effluent | Click to download |
| 6241 | A Purification Strategy for Clinical-Grade Monoclonal Antibody Using Hydrophobic Cation Exchange Chromatography | Click to download |
| 6067 | EDTA Complexometric Titration of Hydroxyapattite Column Effluent | Click to download |
| 5739 | Packing and Slurry-in-Place Procedures for CHT™ Resin using Bio-Rad® InPlace™ Chromatography Columns | Click to download |
| 6418 | Purification of Recombinant Proteins on Nuvia™ cPrime™ Hydrophobic Cation Exchange Resin: A Simple Approach to Method Development | Click to download |
| 2204 | Effective Removal of Negatively Charged Interfering Molecules from Proteins | Click to download |
| 2780 | Purification of Murine IgG1 Using UNOsphere™ S and CHT™ Ceramic Hydroxyapatite Chromatography | Click to download |
| 2524 | Purification of Horse IgG Using Macro-Prep® DEAE and CHT™ Ceramic Hydroxyapatite Type I Resins | Click to download |
| 2774 | Purification of Transgenic Antibody from Corn Seed Using UNOsphere™ S and CHT™ Ceramic Hydroxyapatite Supports | Click to download |
| 2940 | Removal of Aggregate From an IgG4 Product Using CHT™ Ceramic Hydroxyapatite Resin | Click to download |
| 2849 | Protein A Removal from IgG on CHT™ Ceramic Hydroxyapatite Resin | Click to download |
| 2535 | Purification of Recombinant Human Alpha 1-Antitrypsin From Transgenic Milk Using CHT™ Ceramic Hydroxyapatite Resin | Click to download |
| 2575 | Purification of Calcium-Binding Protein using CHT™ Resin | Click to download |
| 2946 | Plasmid DNA Adsorption Behavior on CHT™ Type II Resin | Click to download |
| 2644 | CHT™ Ceramic Hydroxyapatite Resin: Use In Expanded-Bed Adsorption Mode | Click to download |
| 5853 | Chimeric IgG Monoclonal Antibody Purification: A Comparative Study Using CHT™ and CFT™ Chromatography Resins | Click to download |
| 1986 | Effect of pH on Gradient Elution of Proteins on Two Types of CHT™ Ceramic Hydroxyapatite Resin | Click to download |
| 5913 | Separation of Fab and Fc Fragments from mAb Papain Digest on Ceramic Hydroxyapatite and Ceramic Fluoroapatite Resins | Click to download |
| 6720 | Development of a cGMP-Ready Purification Process for Adenovirus Purification (Poster) | Click to download |
| Number | Description | Download |
|---|---|---|
| 6086 | CHT™ Ceramic Hydroxyapatite Resource Guide - available online at www.bio-rad.com/chtguide | Click to download |
Hydrophobic Resins: Macro-Prep® and Bio-Beads™ Matrices
Macro-Prep® Hydrophobic Interaction Chromatography (HIC) Resins are used for protein, polypeptide, enzyme, and nucleic acid purification. These resins are designed specifically for intermediate purification steps to remove host-cell contaminants from partially purified targets. Due to the rigidity and unique surface chemistry of these resins, they are well-suited for HIC operations requiring high throughput and high recovery of target.
| Number | Description | Download |
|---|---|---|
| 1841 | Macro-Prep® HIC Resin | Click to download |
| 1461 | Bio-Beads™ SM-2 Hydrophobic Adsorbents Application Bibliography | Click to download |
| 1942 | Macro-Prep® DEAE Support for Chromatography of BioMolecules | Click to download |
| Number | Description | Download |
|---|---|---|
| LIT486 | Instruction Manual, Macro-Prep® HIC Resins | Click to download |
| LIT195 | Instruction Manual, Bio-Beads™ SM Hydrophobic and Polar Interaction Adsorbents | Click to download |
Affinity Resins: UNOsphere™ Matrix
Ready-to-use activated affinity media and IMAC and protein A resins are available for affinity purification of biomolecules. Protein A resins can be used for different monoclonal antibody (mAb) purification needs. UNOsphere SUPrA™ Resin is designed for process-scale purification of mAbs.
| Number | Description | Download |
|---|---|---|
| 5729 | UNOsphere SUPrA™ Resin | Click to download |
| 6484 | UNOsphere™ Affinity Columns PIS | Click to download |
| Number | Description | Download |
|---|---|---|
| 5728 | Monocolonal Antibody Purification Using UNOsphere SUPrA™ Resin | Click to download |
| 6053 | Application of UNOsphere SUPrA™ Resin, a New Protein A Affinity Chromatographic Support, for Both Laboratory-Scale and Industrial Antibody Purification | Click to download |
| Number | Description | Download |
|---|---|---|
| 10014430 | Instruction Manual, UNOsphere SUPrA™ Affinity Chromatography Media | Click to download |
| 10003245 | Instruction Manual, Profinity™ Epoxide Resin | Click to download |
| 10044307 | Instruction Manual, Bio-Rad® Nuvia™ IMAC Resin | Click to download |