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CHEF DNA Size Standard, 8.3–48.5 kb, Lambda Ladder

CHEF DNA Size Standard, 8.3–48.5 kb, Lambda Ladder #1703707

125 μl, 0.2 μg/μl, PFGE standard, 8.3–48.5 kb, mixed digests of phage lambda (λ), 125 applications
Available Safety Data Sheets
Safety Data Sheet

Safety Data Sheet

Overview

Use CHEF* DNA size standards for size estimation of larger DNA fragments separated by pulsed field electrophoresis.

  • Size range: 8.3–48.5 kb
  • Restriction digested λ DNA resolved into 13 bands
  • Recommended gels: 1.0–1.5% Pulsed Field Certified Agarose or molecular biology grade agarose

Note: Cut a plug from a block into a size that will fit a well of your gel (5–8 plugs per block). The height of the plug should not extend above the well. After placing the plug into a well, seal the well with melted 1.0% low-melt agarose, and allow it to solidify. Store blocks at 4°C.

Pulsed Field Size Standards Size Range
CHEF DNA Size Marker (#170-3605) 0.2–2.2 Mb
CHEF DNA Size Standard (#170-3624) 4.9–98 kb
CHEF DNA Size Standard (#170-3635) 48.5–1,000 kb
CHEF DNA Size Marker (#170-3667) 1–3.1 Mb
CHEF DNA Size Standard (#170-3707) 8.3–48.5 kb

Related Products

More Information

Download the Little Book of Standards for complete product information on all Bio-Rad nucleic acid and protein standards.

*Clamped homogenous electrical field