Biofuel Enzyme Reactions Kit for AP Biology #17001235EDU

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Overview

Enzyme kinetics kit, includes enzyme, substrate, standard, solution, buffers, disposable pipets, tubes, and cuvettes for 32 students; inquiry-driven curriculum; education use only
Safety Data Sheet

Safety Data Sheet

Description

Description

Need more structure?
Try the original Biofuel Enzyme Kit
Help your students to gain a deep understanding of enzyme kinetics by playing the role of bioengineers trying to optimize conditions for cellobiase to break down cellulose, an important step in biofuel production.

Students first extract cellobiase from mushrooms to discover one way that nature uses enzymes. They then generate hypotheses about and conduct experiments to test enzyme function under different environmental conditions—temperature, pH, and concentration—that influence enzyme function. This kit aligns with AP Biology Big Ideas 2 and 4, and gives teachers the flexibility and support to conduct the labs as structured, guided and/or open inquiry investigations.

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Related Categories

Supporting Documents

Online Resources

Specifications

Specifications

Kit contains sufficient materials for 8 student workstations (2–4 students per workstation)
 
Enzyme, cellobiase, 1 ml
1 vial
Substrate, p-nitrophenyl glucopyranoside, 90 mg
1 vial
Standard, p-nitrophenol (1 mM, 4 ml)
1 bottle
2x stop solution, 100 ml
100 ml
10x resuspension buffer, 50 ml
1 bottle
Extraction buffer, 50 ml
1 bottle
Disposable plastic transfer pipets (DPTPs)
40
1.5 ml microcentrifuge tubes
90
15 ml conical tubes
50
1.5 ml standard disposable polystyrene cuvettes
100
Curriculum, including Teacher's Guide and Student Manual
1
Student Instruction Manual, available for download

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Recommended SKUs