STEM Electrophoresis Kit

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STEM Electrophoresis Kit

Overview

STEM Electrophoresis Kit Quick Guide

Download the STEM Electrophoresis Kit Quick Guide for the color version of the student protocol.

 

STEM Electrophoresis Kit Flowchart

Lab Preparation Checklist
  STEM Electrophoresis
Classroom Kit
166-5090EDU
STEM Electrophoresis
Teacher Demonstration Kit
166-5080EDU
Kit Contents (sufficient materials for 8 student workstations with 2–4 students per workstation)
Store at room temperature
Dye extraction solution, 25 ml 1 1
Blue 1 reference dye, 150 µl 1 1
Yellow 5 reference dye, 150 µl 1 1
Yellow 6 reference dye, 150 µl 1 1
Red 40 reference dye, 150 µl 1 1
Electrophoresis buffer, 50x TAE, 100 ml 1 1
Molecular biology grade agarose, 5 g 1 1
Microcentrifuge tubes, 2 ml 72 72
Hinged plastic boxes 4 1
Paper clips 16 4
Black alligator clip leads 8 2
Red alligator clip leads 8 2
8-well combs 8 2
Curriculum (includes teacher guide, student manual, and graphic quick guide) 1 1
Required Accessories Not Included in Kit
9 V batteries (3–5 per workstation) 24–40 6–10
Plastic rulers or plastic cards to cut gels 8 2
2–20 µl adjustable-volume micropipets or 10 µl fixed-volume micropipets 8 2
2–200 µl pipet tips, 1,000/bag 1 1
Eyedroppers or
100–1,000 µl adjustable-volume micropipets
  or disposable plastic transfer pipets (DPTPs)

8
40

2
10
100–1,000 µl pipet tips* 1 bag 1 bag
Permanent markers 8 2
Plastic cups or small beakers 32 8
Microwave oven or hot plate 1 1
Distilled water 3 liters 1 liter
500 ml Erlenmeyer flask for microwaving agarose 1 1
Candies with a variety of color coatings Variable Variable
Balance 1 1
Recommended (Optional) Accessories
Microcentrifuge or mini centrifuge 1 1
Digital camera for imaging gels 1 1
Microcentrifuge tube rack 8 1–2
* Not needed if using DPTPs or eyedroppers instead of 100–1,000 µl adjustable-volume micropipets.
Key Kit Features
  • Inquiry-based hands-on laboratory
  • Kit can be completed in two 45-min laboratory sessions
  • Visible results
  • STEM integration
Curriculum Fit
STEM Curriculum Chart

Biotechnology and STEM go hand in hand. Science in the 21st century is driven by the integration of science, technology, engineering, and mathematics (STEM). Citizens and scientists alike are required to understand these fundamentals in order to make decisions about issues ranging from personal healthcare solutions to global energy challenges. Biotechnology Explorer™ kits help you teach more efficiently by integrating STEM content into a single lab so that you can focus on what matters most — teaching.

Build a horizontal gel electrophoresis chamber and integrate science, technology, engineering, and mathematics (STEM) to showcase how these disciplines are combined to develop tools to solve problems.
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  • Ordering
  • Accessories
  • Refills
  • Documents

Description

Great ideas stem from inquiry!
Science, technology, engineering, and mathematics (STEM) are essential to each other in order to develop products to meet our needs. In fact, it is difficult to separate each of these disciplines from one another when teaching real-world applications. The STEM electrophoresis kit is a unique introductory electrophoresis laboratory with true integration of STEM through the assembly of a horizontal agarose gel electrophoresis cell. Perform actual dye electrophoresis using the STEM electrophoresis kit with the IDEA kit — inquiry dye electrophoresis activity.

Features and Benefits

  • Inquiry-based hands-on laboratory
  • Kit can be completed in two 45-min laboratory sessions
  • Visible results
  • STEM integration

Applications and Uses

This kit offers learning opportunities for all levels of instruction:

  • For secondary and college level instruction, students will learn about the various components of a horizontal gel electrophoresis cell and what their critical properties are in order to achieve optimal separation results
  • For middle school instruction, students will get their first experience with a key biotechnology technique

More Information

  • The IDEA kit — inquiry dye electrophoresis activity is designed to run with the STEM electrophoresis kit
  • The STEM electrophoresis teacher demonstration kit provides sufficient materials for 2 student workstations
  • The STEM electrophoresis classroom kit provides sufficient materials for 8 student workstations with up to 4 students per workstation
  • The STEM electrophoresis engineering module is configured for 2 student workstations
  • Download this pop culture activity for a fun way to use the IDEA/STEM Kits in your classroom:
    Using Pop Culture in Your Science Class (MS PowerPoint, 5 MB)


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STEM Electrophoresis Teacher Demonstration Kit

1665080EDU
Introductory kit, includes IDEA kit reagent refill pack (1665076EDU [4 reference dyes, dye extraction solution, microcentrifuge tubes]), TAE buffer, agarose, electrophoresis cell, electrodes, electrical leads, combs, curriculum, for 2 student workstations; education use only

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$123.75 
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STEM Electrophoresis Classroom Kit

1665090EDU
Introductory kit, includes IDEA kit reagent refill pack (1665076EDU [4 reference dyes, dye extraction solution, microcentrifuge tubes]), TAE buffer, agarose, electrophoresis cell, electrodes, electrical leads, combs, curriculum, for 8 student workstations; education use only

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$342.50 
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$274.00  
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STEM Electrophoresis Kit Starter Pack

1665095EDU
Introductory kit, includes IDEA kit reagent refill pack (1665076EDU [4 reference dyes, dye extraction solution, microcentrifuge tubes]), TAE buffer, agarose, electrophoresis cell, electrodes, electrical leads, combs, fixed-volume pipets, tips, curriculum, for 8 student workstations; education use only

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$536.25 
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$429.00  
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Accessories

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10 µl Fixed-Volume Micropipet

1660512EDU
Pkg of 1, color-coded green micropipet, accommodates standard pipet tips; education use only

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$43.33 
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$26.00  
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Classroom 2–20 μl Digital Micropipet

1660551EDU
Pkg of 1, 2–20 μl, adjustable-volume micropipet, includes digital dial, tip ejector, accommodates standard pipet tips; education use only

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$165.00 
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Professional Adjustable-Volume 2–20 μl Digital Micropipet

1660506EDU
Pkg of 1, 2–20 μl adjustable-volume micropipet, includes digital dial micrometer, tip ejector, accommodates standard pipet tips, fully autoclavable; education use only

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$337.50 
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BR-35 Pipet Tips

2239035EDU
Pkg of 1,000, 2–200 µl standard pipet tip, bulk, autoclavable, yellow; education use only

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$43.00 
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Refills

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STEM Electrophoresis Engineering Module

1665085EDU
Consumables refill module, includes hinged plastic box, electrodes, electrical leads, combs, for 2 student workstations; education use only

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$71.25 
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$57.00  
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IDEA Kit Reagent Refill Pack

1665076EDU
Consumables refill kit for use with IDEA kit (1665075EDU) or STEM electrophoresis kit (1665080EDU, 1665090EDU), includes reference dyes, extraction buffer, microcentrifuge tubes; education use only

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$33.75 
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$27.00  
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IDEA Kit — Inquiry Dye Electrophoresis Activity

1665075EDU
Introductory agarose gel electrophoresis kit, includes reference dyes, extraction buffer, microcentrifuge tubes, agarose, TAE, curriculum; education use only

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$68.75 
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$55.00  
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50x Tris/Acetic Acid/EDTA (TAE)

1660742EDU
100 ml, TAE nucleic acid electrophoresis buffer solution, pH 8.0; education use only

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$18.75 
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$15.00  
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Documents

Number Description Options
6288 Instruction Manual, STEM Electrophoresis Kit Quick Guide, Biotechnology Explorer™, Rev A Click to download
1665086EDU Instruction Manual, STEM Electrophoresis Kit, Biotechnology Explorer™, Rev A Please login to download this document

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