iScript™ RT-qPCR Sample Preparation Reagent

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iScript RT-qPCR Sample Preparation Reagent

Overview

Performance

iScript RT-qPCR sample preparation reagent generates linear results over varying input cell amounts. HeLa cells were treated with each reagent (125, 25, 5, and 1 cells/µl) according to the recommended guidelines and analyzed for GAPDH expression levels using iScript cDNA synthesis kit and iQ SYBR® Green supermix on the CFX96 real-time PCR detection system. iScript RT-qPCR sample preparation reagent delivers excellent efficiency and reproducibility for the detection of GAPDH from cell extracts. GAPDH efficiency = 94.4%, R2 = 0.996. RFU, relative fluorescence units.

18S rRNA, β-Tubulin, and C-Myc Expression Levels
iScript RT-qPCR Sample Preparation Reagent

PureZOL RNA Isolation Reagent

iScript RT-qPCR sample preparation reagent generates earlier CTs compared to PureZOL RNA isolation reagent. HeLa cells were treated with each reagent (125 cells/µl) according to the recommended guidelines and analyzed for 18S rRNA (), β-tubulin (), and c-Myc () expression levels using iScript cDNA synthesis kit and iQ SYBR® Green supermix on the CFX96 real-time PCR detection system. iScript RT-qPCR sample preparation reagent delivers earlier CTs as compared to PureZOL treatment. Average CT values for triplicate reactions are shown for each gene target (inset tables). RFU, relative fluorescence units.

Bio-Rad's iScript RT-qPCR sample preparation reagent delivers efficient cell lysis and RNA stabilization for sensitive qPCR without RNA purification.
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Description

iScript™ RT-qPCR sample preparation reagent provides a simple and rapid procedure (<10 min) for isolating total RNA from cultured cells to use for RT-qPCR. This reagent delivers efficient cell lysis and RNA stabilization for sensitive qPCR without traditional RNA sample preparation.

Key Features and Benefits

  • Have ease of use and simplicity — one-buffer formulation contains all of the necessary components to perform cell lysis and RNA stabilization in 5–10 min
  • Benefit from superior compatibility with qPCR directly from cells — sensitive detection of high-, medium-, and low-copy gene targets directly from cell lysates using real-time qPCR when combined with iScript reverse transcription and qPCR reagents
  • Maximize data from very few cells — reagent enables multiplex real-time detection of up to 4 targets from as few as 10 cells
  • Extend isolated RNA shelf-life — isolated RNA is stable for up to 6 months when frozen
  • Use with different cell types — compatible with both suspension and adherent cell cultures

Specifications

Storage at –20°C Guaranteed for 12 months at –20°C in a constant temperature freezer (multiple freeze thaws not recommended)

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iScript™ RT-qPCR Sample Preparation Reagent

1708898
100 reactions, 10 ml, contains RNA stabilizers

List Price: 
 
$175.00 
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iScript™ RT-qPCR Sample Preparation Reagent

1708899
500 reactions, 5 x 10 ml, contains RNA stabilizers

List Price: 
 
$613.00 
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Documents

Number Description Options
6090 Amplification Reagents and Plastics Brochure Click to download [ Add to Cart (Free) ]
10013596 Instruction Manual, iScript RT-qPCR Sample Preparation Reagent, Rev A Click to download
6763 Gene Expression Real-Time PCR Workflow Flier Click to download

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