iScript™ RT-qPCR Sample Preparation Reagent

iScript™ RT-qPCR sample preparation reagent provides a simple and rapid procedure (<10 min) for isolating total RNA from cultured cells to use for RT-qPCR. This reagent delivers efficient cell lysis and RNA stabilization for sensitive qPCR without traditional RNA sample preparation.

Key Features and Benefits

• Have ease of use and simplicity — one-buffer formulation contains all of the necessary components to perform cell lysis and RNA stabilization in 5–10 min
• Benefit from superior compatibility with qPCR directly from cells — sensitive detection of high-, medium-, and low-copy gene targets directly from cell lysates using real-time qPCR when combined with iScript reverse transcription and qPCR reagents
• Maximize data from very few cells — reagent enables multiplex real-time detection of up to 4 targets from as few as 10 cells
• Extend isolated RNA shelf-life — isolated RNA is stable for up to 6 months when frozen
• Use with different cell types — compatible with both suspension and adherent cell cultures