High Resolution Melt Analysis

Image
precision-melt-supermix-detail.jpg

Overview

This EvaGreen qPCR supermix is specially formulated for high-resolution melt analysis and optimized for specificity and accurate discrimination of challenging SNPs. Compatible with most HRM-enabled real-time PCR systems.
Read full description Ordering Information

Description

Description

Precision Melt Supermix is a superior high-resolution melt (HRM) supermix delivering optimal discrimination for numerous applications including:

  • Genotyping
  • Mutation analyses
  • Methylation studies
  • Species identification
  • Epigenetic analyses
  • De novo SNP discovery

Key Features, Benefits and Applications of Precision Melt Supermix

  • Sensitive and specific discrimination of class I–IV single nucleotide polymorphisms (SNPs) across a broad range of amplicons
  • Ideal solution for insertion/deletions >6 base pairs
  • Accurate detection of the percent of CpG methylation status for epigenetic studies
  • Ideal for mutation screening of small mutations or using a primer walking approach for larger regions
  • Ideal cost-saving application for upstream and downstream next generation sequencing pre-screens and post-validations
  • Exceptional room temperature stability for high-throughput HRM studies
  • Optimized formulation containing EvaGreen dye delivers robust PCR and HRM performance

Instrument Compatibility

For use with all HRM-ready real-time PCR systems; dye calibration may be required.

Packaging Options

Number of 20 µl Reactions
Volume Catalog Number
200 2 ml 1725110
1,000 10 ml
1725112

Related Products

Related Categories

Online Resources

Specifications

Specifications

Storage at –20°C 
Guaranteed for 6 months at –20°C in a constant temperature freezer (multiple freeze thaws not recommended)

Ordering

Ordering

items

Use the filters below to refine results!

172-5110_tn.jpg
1725110
2 x 1 ml, 2x real-time PCR mix, contains dNTPs, iTaq™ DNA polymerase, MgCl2, EvaGreen dye, stabilizers, for 200 x 20 µl reactions
172-5112_tn.jpg
1725112
10 x 1 ml, 2x real-time PCR mix, contains dNTPs, iTaq™ DNA polymerase, MgCl2, EvaGreen dye, stabilizers, for 1,000 x 20 µl reactions

Documents

Documents

Number Description Options
10022094
Instruction Manual, Precision Melt Supermix, Rev B
6137
Precision Melt Supermix Product Information Sheet, Rev A
6090
Amplification Reagents and Plastics Brochure
[ Add to Cart (Free) ]