Overview
5–33 cm ID Columns
Foresight Pro Chromatography Columns are GMP ready columns specifically designed for use in all biopharmaceutical manufacturing applications. The columns have a sanitary design with minimal dead space and effective cleanability, and connections to pumping systems are made via standard 3/4" Tri-Clamp fittings.
Prepacked columns with Nuvia Media are available in a range of column diameters and bed heights. These columns are shipped in 20% ethanol and/or salt, as applicable to the resin. (See specification table.)
Prepacked Column Features and Benefits
- GMP ready columns — validated and ready to use
- Excellent performance — data include asymmetry, HETP, endotoxin, and bioburden
- Robust column hardware design maintains a packed bed
- Columns maintain performance criteria after shipping and cycling
- All wetted parts meet USP Class VI requirements of USP <88>
- Standard Tri-Clamp connections — plug and play
- Columns are packed in a controlled ISO Class 7 cleanroom
- Range of column diameters and bed heights — 5–13 cm ID and 10 or 20 cm bed heights
- Resin reference sample included with each column
- Regulatory support file available
Nuvia aPrime 4A Media Applications
- Polishing of monoclonal antibodies (mAbs) in flow-through mode and capture mode
- Host cell protein (HCP) removal
- DNA and endotoxin clearance
- Virus clearance and removal
- Polishing of recombinant proteins
- Aggregate removal
Nuvia cPrime Media Applications
- Capture and polishing of mAbs
- Recombinant protein purification
- Biotherapeutic capture at high salt concentrations
- Aggregate and charge variant removal
- Post Protein A polishing steps
- Process-scale purification of biotherapeutics across a wide pH range
Packing Quality
The performance of the Foresight Pro Columns was evaluated before and after International Safe Transit Association (ISTA) testing. As shown in Figure 1, the columns maintained performance criteria, indicating excellent stability during shipping.
Cycling Data
The performance of the Foresight Pro Columns was evaluated for flow stability. The column went through a series of cycles. Figures 2 and 3 show that the columns maintained performance criteria, indicating a robust packed bed.
Figure 4 shows the linear relationship between pressure and flow rate for Foresight Pro Columns packed with Nuvia Mixed-Mode Media. The data indicate that column pressure remains below 3 bar at the operating linear velocity of250 cm/hr for the Foresight Pro Nuvia cPrime Column.
Non-Product Contact Material Summary
| Element | Material |
|---|---|
| Top and bottom supporting plates | Polypropylene |
| Holding bars | Polyamide or 304 stainless steel |
Product Contact Material Summary
| Element | Material | USP <88> Class | CFR 21 177 | Animal Origin |
|---|---|---|---|---|
| Column body | Glass (5 cm ID columns) | N/A | N/A | N/A |
| Column body | Acrylic (8, 10, 13 cm ID columns) | Class VI | 177.1010 | Animal-free |
| Flow distributors | Polypropylene | Class VI | 177.1520 | Animal-free |
| Inlet and outlet connectors | Polypropylene | Class VI | 177.1520 | Animal-free |
| Tubing connections | Reinforced elastomer tubing | Class VI | 177.2600 | Animal-free |
| Tri-Clamp Gasket and O-rings | Ethylene propylene diene monomer (EPDM) | Class VI | 177.2600 | Animal-free |
| Bed support screens | Polyamide | Class VI | 177.1500 | Animal-free |
Related Products
- Nuvia cPrime Media
- Nuvia aPrime 4A Media
- Nuvia wPrime 2A Media
Related Categories
- Nuvia Mixed-Mode Resins
- Foresight Pro Chromatography Columns, Nuvia Ion Exchange Resins
- Foresight Pro Chromatography Columns, CHT™ Resins
- Foresight Prepacked Chromatography Filter Plates & Columns