One-Step RT-ddPCR Advanced Kit for Probes #1864022

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Overview

500 x 20 µl reaction kit, for absolute quantification of target RNA in the QX200™AutoDG™/QX200™/QX100™ Droplet Digital™ PCR Systems

Safety Data Sheet

Safety Data Sheet

Description

Description

Use this one-step reverse transcription digital PCR supermix to achieve improved efficiency, specificity, and sensitivity during precise RNA target quantification with Droplet Digital™ PCR (ddPCR™).

Key Benefits

  • Absolute quantification by Droplet Digital PCR in a convenient single-reaction format
  • Contains all components required for hydrolysis probe–based RT-ddPCR except primers, probe(s), and template
  • Limits nonspecific PCR amplification
  • Optimized enzyme blend enables partitioning of RNA samples into droplets while keeping the enzymes inactive until the reverse transcription reaction is performed at 50°C
  • Contains RNase inhibitor to protect the RNA throughout the entire workflow

Applications and Uses

  • Absolute quantification
  • Gene expression analysis
  • Viral load detection
  • Pathogen detection

Packaging Options

Number of 20 µl Reactions Catalog Number
200 186-4021
500 186-4022

 

Additional Reagents

 

Bio-Rad offers additional consumables for completing your digital PCR workflow:

Additional Equipment

This digital PCR supermix requires the use of one or more of the following instruments and accessories:

Related Products

Bio-Rad offers additional digital PCR supermixes including:

More Information

This ddPCR Supermix for Residual DNA Quantification is optimized for use with Droplet Generation Oil for Probes on the QX200™ Droplet Digital™ PCR System and QX200™ AutoDG™ Droplet Digital™ System.

Specifications

Specifications

Storage at –20°C
Up to 12 months (refer to expiration date)
Template compatibility
RNA