Overview
500 x 20 µl reaction kit, for absolute quantification of target RNA in the QX600/QX200 Droplet Digital™ PCR Systems
Safety Data Sheet
Description
Description
Use this one-step reverse transcription digital PCR supermix to achieve improved efficiency, specificity, and sensitivity during precise RNA target quantification with Droplet Digital™ PCR (ddPCR™).
Key Benefits
- Absolute quantification by Droplet Digital PCR in a convenient single-reaction format
- Contains all components required for hydrolysis probe–based RT-ddPCR except primers, probe(s), and template
- Limits nonspecific PCR amplification
- Optimized enzyme blend enables partitioning of RNA samples into droplets while keeping the enzymes inactive until the reverse transcription reaction is performed at 50°C
- Contains RNase inhibitor to protect the RNA throughout the entire workflow
Applications and Uses
- Absolute quantification
- Gene expression analysis
- Viral load detection
- Pathogen detection
Packaging Options
| Number of 20 µl Reactions | Catalog Number |
| 200 | 186-4021 |
| 500 | 186-4022 |
Additional Reagents
Bio-Rad offers additional consumables for completing your digital PCR workflow:
- Digital PCR consumables
- PrimePCR™ ddPCR™ Assays
Additional Equipment
This digital PCR supermix requires the use of one or more of the following instruments and accessories:
- QX600 AutoDG Droplet Digital PCR System
- QX600 Droplet Digital PCR System
- Automated Droplet Generator
- QX200™ Droplet Generator
- QX200™ Droplet Reader
- PX1™ PCR Plate Sealer
- C1000 Touch™ Thermal Cycler with 96–Deep Well Reaction Module
Related Products
Bio-Rad offers additional digital PCR supermixes including:
- ddPCR™ Supermix for Probes (No dUTP)
- ddPCR™ Supermix for Probes
- Residual DNA Quantification Supermix
- QX200™ ddPCR™ EvaGreen Supermix
More Information
This ddPCR Supermix for Residual DNA Quantification is optimized for use with Droplet Generation Oil for Probes on the QX600 or QX200™ Droplet Digital™ PCR System and QX600 or QX200™ AutoDG™ Droplet Digital™ System.