ddPCR and RT-PCR Assays for SARS-CoV-2 Variant Surveillance


With the emergence of SARS-CoV-2 variants that may confer an evolutionary advantage to the virus, the need for fast, sensitive, and reliable tests to monitor for variants is essential both for tracking purposes and sequencing prioritization.

The SARS-CoV-2 Variant Assays are predesigned mutation detection assays targeting the most prominent variants known to date. These are single-tube duplex assays designed to support detection of a specific mutation in the FAM channel and wild-type sequence in the HEX channel using either Droplet Digital PCR (ddPCR™) or RT-PCR.

New assays are expected to be made available as new variants emerge.

The SARS-CoV-2 Variant Assays are for research use only. Not for use in diagnostic procedures.

Featured ProductSARS-CoV-2 ddPCR™ and RT-PCR Assays

Single and Multiple Mutation Assays for
SARS-CoV-2 Variants

Profile mutations associated with SARS-CoV-2 variants using new
single and multiple mutation assays designed for ddPCR™ and RT-qPCR.
For research use only (RUO).

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SARS-CoV-2 Variant Assay Protocols, Kits, and Reagents

SARS-CoV-2 Variant Assay Protocols

SARS-CoV-2 Assay Kits and Reagents

  • SARS-CoV-2 Droplet Digital PCR (ddPCR) Kit

    SARS-CoV-2 Droplet Digital PCR (ddPCR) Kit

    The Bio-Rad SARS-CoV-2 ddPCR Kit is a powerful diagnostic tool in the battle against COVID-19, with sensitivity and precision beyond that of qPCR assays.

  • Reliance SARS-CoV-2 RT-PCR Assay Kit

    Reliance SARS-CoV-2 RT-PCR Assay Kit

    The Reliance SARS-CoV-2 RT-PCR Assay Kit is a molecular in vitro diagnostic test targeting the virus nucleocapsid N1 and N2 genes to detect the coronavirus specifically.

  • One-Step RT-ddPCR Advanced Kit for Probes

    One-Step RT-ddPCR Advanced Kit for Probes

    Use this one-step reverse transcription digital PCR supermix to achieve improved efficiency, specificity, and sensitivity with precise RNA target quantification using Droplet Digital PCR (ddPCR).

  • Reliance One-Step Multiplex RT-qPCR Supermix

    Reliance One-Step Multiplex RT-qPCR Supermix

    Use Reliance One-Step Multiplex Supermix for sensitive amplification of up to 5 targets in a single reaction with challenging templates, difficult targets, or PCR inhibitors.

  • SARS-CoV-2 Standard

    SARS-CoV-2 Standard

    The SARS-CoV-2 Standard contains synthetic RNA transcripts of 5 SARS-CoV-2 gene targets and Human Genomic DNA.

  • SARS-CoV-2 Negative

    SARS-CoV-2 Negative

    The SARS-CoV-2 Negative contains genomic DNA at 75,000 copies/ml formulated in a synthetic matrix.

Custom Assays for ddPCR and RT-PCR

Choose from a large catalog of predesigned, validated ddPCR and qPCR assays or design your own custom assays.

  • Expert Design ddPCR assays are designed for optimal performance and manufactured to reduce false positives.
  • PrimePCR Assays and panels are carefully designed and validated to yield reliable qPCR data.
  • ddPCR Expert Design Assays

    ddPCR Expert Design Assays

    ddPCR Expert Design Assays are designed specifically for Droplet Digital PCR by the experts that developed the technology.

  • PrimePCR Assays and Panels

    PrimePCR Assays and Panels

    Experimentally validated real-time PCR PCR primer and probe assays for mutation detection, gene expression, copy number variation. Design your own custom assays and panels.

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More COVID-19 Surveillance Solutions

COVID-19 surveillance is needed to better inform public health decisions. Obstacles to surveillance work include large sample numbers, supply shortages, and complex sample types. Bio-Rad offers a range of solutions for COVID-19 surveillance.

Learn More about our COVID-19 Surveillance Solutions »