Better Western Blotting Guide
Tips, Techniques, and Technologies from the Western Blotting Experts at Bio‑Rad Laboratories
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The Western Blot Doctor is a self-help guide developed by Bio-Rad researchers that enables you to identify and troubleshoot western blotting problems. Comprehensive solutions and suggestions are provided to help solve your particular western blotting challenges.
To start troubleshooting your western blotting problems, choose the type of problem you are experiencing.
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Protein Blotting Methods, Protein Blotting Equipment (Cells and Power Supplies), Membranes and Blotting Papers, Transfer Conditions, Protein Blotting Detection and Imaging, Transfer Buffers
Good western blotting results begin with high-quality protein electrophoresis results. For detailed descriptions of the best practices for optimal protein separation by polyacrylamide gel electrophoresis, visit the Protein Electrophoresis section.
Download individual western blotting protocols in PDF format in the Protocols tab below.
Videos
Documents
TEST
| 6359 | Avoiding Housekeeping Protein Detection Saturation Protocol, Rev A | Click to download |
| 6361 | Determining the Appropriate Film Exposure Time Protocol, Rev A | Click to download |
| 6362 | Determining the Appropriate Sample Load for Western Blots Protocol, Rev A | Click to download |
| 6363 | Determining the Appropriate Sample Load When Using a Stain-Free V3 Western Workflow™ Protocol, Rev A | Click to download |
| 6366 | Validating the Expression Consistency of a Housekeeping Protein Protocol, Rev A | Click to download |
| 6376 | General Protocol for Western Blotting Protocol, Rev A | Click to download |
| 6390 | General V3 Western Workflow Blotting Protocol, Rev A | Click to download |