Kovacs Reagent, for indole detection #3555313

Use Kovacs Reagent for the detection of bacteria, such as coliforms including E. coli, that under aerobic conditions are able to split tryptophan into indole and α-aminopropionic acid. The reagent contains para-dimethylaminobenzaldehyde (DMAB), and concentrated hydrochloric acid. After growth in a tryptophan-containing broth such as Schubert Broth (3569634) or BGBLB (Brilliant Green Bile Lactose Broth) (3578024), addition of Kovacs Reagent results in an immediate local red color in indole-producing cultures, due to DMAB complexing with indole.

Related Products

• Schubert Broth (modified by Fennel) (3569634)
• BGBLB (Brilliant Green Bile Lactose Broth) (3578024)
• Tryptose Lauryl Sulfate Broth (3564774)
• BCP (Bromocresol Purple) Lactose Broth (3553414)
• MUD and MUG Microplates
• RAPID'E.coli 2 Agar and RAPID'Enterococcus Agar (3554409) for Water Testing; high color-contrast agar methods for direct counts, with no confirmation steps

Related Categories

• Food Safety Testing
• Chromogenic Media
• Standard Media for Water Testing
• Fluorogenic Media for Water Testing
• XplOrer64™ System and CheckN'Safe™ Kits for real-time monitoring of recreation waters

Supporting Documents

• Food Safety and Quality Diagnostics — Guide for Products in Food Testing
• Water Safety Booklet — Guide for Products in Water Testing