Overview
Use the ddPLEX EGFR/KRAS/BRAF Mutation Detection Kit to detect 37 key non-small cell lung cancer (NSCLC) mutations. This kit provides all necessary reagents for detecting these variants and their wild-type counterparts using cell-free DNA (cfDNA) in plasma and/or tumor tissue samples, enabling calculation of variant allele frequencies.
When paired with Bio-Rad’s QX600TM Droplet DigitalTM PCR (ddPCRTM) System* and using the recommended controls and guidelines, labs can process up to 42 unknown samples per plate and complete sample loading to data generation in under 8 hours.
* For Research Use Only. Not for use in diagnostic procedures.
Features and Benefits
- High sensitivity: detect established NSCLC mutations at ≤ 0.1% VAF** in a single well
- Accelerated turnaround: achieve same-day results with a streamlined workflow
- Versatile sample input: compatible with plasma or formalin-fixed paraffin embedded (FFPE) samples for greater flexibility
- Built-in quality assurance: includes an internal control
- Enhanced detection power: patented wild-type suppression chemistry boosts sensitivity
- Reliable performance: powered by ddPCR technology using the QX600 ddPCR System and QX Manager Software (SE v2.1 or higher) for exceptional reproducibility and specificity
** Variant Allele Frequency
Product Contents
This kit includes the following components for 120 reactions.
- 12023484 - ddPLEX EGFR/KRAS/BRAF Mutation Detection Assays - Qty 1
- ddPLEX EGFR/KRAS/BRAF Total Quantification Assay
- ddPLEX EGFR/KRAS/BRAF Mutant Assay
- ddPLEX Enhancer
- ddPLEX Internal Control
- 12023522 - ddPLEX EGFR/KRAS/BRAF Positive Control – Qty 1
- 12005909 - ddPCR Multiplex Supermix – Qty 1
- 12012171 - Dithiothreitol (DTT) – Qty 1
The four components listed above can also be purchased individually.
Configuration Options
- The ddPLEX EGFR/KRAS/BRAF Mutation Detection Kit should be used with the QX600 ddPCR System and either the Manual Droplet Generator or Automated Droplet Generator.
Key Droplet Generation & Reader Equipment, Consumables and Reagents
| Description | Catalog Number |
|---|---|
| QX600 Droplet Reader | 12013328 |
| Automated Droplet Generator | 1864101 |
| QX200 Manual Droplet Generator | 1864002 |
| PTC Tempo Thermal Cycler | 12015392 |
| PCR Plate Heat Seal | 1814040 |
| PX1 PCR Plate Sealer | 1814000 |
| ddPCR Plates 96-well Semi-skirted | 12001925 |
| ddPCR Droplet Reader Oil | 1863004 |
| ddPCR Buffer Control for Probes (2X) | 1863052 |
Reagents & Consumables for Automated Droplet Generation Only
| Description | Catalog Number |
|---|---|
| Automated Droplet Generator Oil for Probes | 1864110 |
| DG32 Automated Droplet Generator Cartridges | 1864108 |
| Pipet Tips for Automated Droplet Generator | 1864120 |
| Pipet Tip Bins for Automated Droplet Generator | 1864125 |
Reagents & Consumables for Manual Droplet Generation Only
| Description | Catalog Number |
|---|---|
| Droplet Generator Oil for Probes | 1863005 |
| Droplet Generator DG8 Cartridge Holder | 1863051 |
| DG8 Cartridges for QX100/QX200 Droplet Generator | 1864008 |
| DG8 Gaskets for QX100/QX200 Droplet Generator | 1863009 |
Related Products
- BRAF V600 ddPCR Multiplex Mutation Screening Kit
- KRAS G12/G13 ddPCR Multiplex Mutation Screening Kit
- EGFR Exon19 Deletions ddPCR Multiplex Mutation Kit
- ddPLEX ESR1 Mutation Detection Kit
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Related Categories
For Research Use Only. Not for use in diagnostic procedures.