VINEO Brettanomytest PCR Kit

VINEO Brettanomytest PCR Kit

Overview

PCR detection of Brettanomyces bruxellensis in wine.

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Description

Description

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During the wine manufacturing process, there are two steps of fermentation, alcoholic fermentation then malolactic fermentation. During alcoholic fermentation, yeast transforms sugar into alcohol. Before fermentation, Saccharomyces is present in the grape juice, as well as other yeasts such as Brettanomyces bruxellensis, commonly known as Brett. After alcoholic fermentation, B. bruxellensis can still be present in the matrix.

The presence of B. bruxellensis is an important issue, as it has been shown that its growth is linked to the concentration of 4-ethylphenol, which imparts a strong off-taste, leading to consumer rejection of the wine. To avoid wine spoilage by B. bruxellensis, some preventative actions can be taken such as racking, clarification, and sulfiting. However, if Brett spoilage has already begun, the winemaker must undertake additional remedial steps such as filtration or heat treatment, increasing production cost. If the concentration of ethylphenol is too high, the lot may have to be destroyed.

Rapid Results

Bio-Rad has developed a new tool for the sensitive, selective, and rapid quantification of B. bruxellensis. The VINEO Brettanomytest Kit monitors Brettanomyces risk. The VINEO method is based on real-time PCR technology and entails two simple steps: DNA extraction followed by detection and quantification of the yeast. Current microbiology culture methods can take 7–14 days before results are available, whereas results are available the same day of analysis with the VINEO Kit. The included software automatically provides an easy interpretation of the results.

Ordering

Ordering

items

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3548100
Kit for 96 tests, includes 100 ml lysis solution, 25 ml elution buffer, 100 ml 100x wash solution, 10 ml 1x wash solution, 96 purification columns, 192 collection vials
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3548101
Kit for 96 tests, includes 0.55 ml fluorescent probes, 2 x 2.2 ml amplification mix, 0.5 ml each positive and negative PCR controls
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3600037

Modular thermal cycler platform, includes C1000 Touch Thermal Cycler chassis, CFX96 Deep Well Optical Reaction Module, cables. To be used with CFX Manager Software, Industrial Diagnostic Edition.

Equipment

Equipment

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3600037

Modular thermal cycler platform, includes C1000 Touch Thermal Cycler chassis, CFX96 Deep Well Optical Reaction Module, cables. To be used with CFX Manager Software, Industrial Diagnostic Edition.

Accessories

Accessories

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TLS0851

Pkg of 120, thin-wall polypropylene 8-tube strips for PCR and low-volume reactions, white (960 tubes)

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TCS0803 TN
TCS0803

Pkg of 120, 8-cap strips for 0.2 ml tubes and plates for PCR and optical reactions, ultraclear

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TCS0803XTU
Pkg of 120, 8-cap strips for 0.2 ml tubes and plates for PCR and optical reactions, ultraclear (960 caps)
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MLL4851
Pkg of 50, white PCR plate, 8 x 6 format, one-component polypropylene, can be cut for fewer samples
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MLL4851XTU
Pkg of 50, white PCR plate, 8 x 6 format, one-component polypropylene, can be cut for fewer samples
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MLL9651
Pkg of 25, white PCR plate, one-component polypropylene, can be cut for fewer samples
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MLL9651XTU
Pkg of 25, white PCR plate, one-component polypropylene, can be cut for fewer samples

Documents

Documents

Number Description Options
FSD_881112
VINEO™ Brettanomytest PCR Kit, user guide (English, French, Spanish, Italian)
6890
VINEO Brettanomytest PCR Test Kit
FSD_881182
VINEO™ Extract DNA Kit, , user guide (English, French, Spanish, Italian)
PF_173
CFX Manager™ Software, Industrial Diagnostic Edition, Quick Guide, Rev B
PF_236
Brettanomyces, or Management of a Contaminant Micro-organism, Rev A