Hard-Shell® 96-Well PCR Plates, low profile, semi-skirted, green/clear #HSL9641



Pkg of 25, green shell/clear well PCR plate, rigid 2-component design



Hard-Shell Low-Profile 96-Well Semi-Skirted PCR Plates are compatible with many cyclers, including Applied Biosystems 7500 fast and ViiA 7 fast instruments and many of Bio-Rad’s thermal cyclers and real-time PCR detection systems. Hard-Shell Plates are specifically designed to withstand the stresses of heat sealing, thermal cycling, and robotic handling. The patented* two-component microplate design provides superior stability and flatness, allowing precise positioning for automation. The low-profile wells are optimized for low-volume reactions and fast PCR.

Key Features and Benefits

  • Semi-skirted — compatible with Applied Biosystems 7500 fast and ViiA 7 fast instruments
  • Highly versatile design — provides compatibility with many thermal cyclers and real-time PCR detection systems, including the Bio-Rad C1000 Touch and CFX Real-Time PCR Systems
  • Extremely uniform wells — reduce well-to-well variability in optical assays performed in real-time PCR
  • Black lettering — facilitates well identification
  • Rigid shell — ideal for heat sealing methods with instruments such as the PX1™ PCR Plate Sealer

Plate Dimensions

Low Profile 96-well Semi-skirted PCR Plate Dimensions

Not drawn to scale.
All dimensions in mm, unless otherwise noted.

Microplate Dimensions Hard-Shell® Low-Profile 96-Well Semi-Skirted PCR Plates (HSL-9xxx)
Length at base plane 127.76 mm
Width at base plane 85.48 mm
Height overall 15.51 mm
Well depth 14.41 mm
Well diameter at opening 5.46 mm
Well diameter at bottom of conical section 3.00 mm
Well volume 200 µl
Well spacing 9.00 mm
Well angle 17.0 °
Well offset  
Left edge to well A1 14.38 mm
Top edge to well A1 11.24 mm
Center of A1 to center of A12 99.00 mm
Center of A1 to center of H1 63.00 mm

Additional Details

  • White-well option for increased fluorescent signal strength
  • Color-coded skirt options with clear and white wells
  • Low-cost, user-readable bar code option for convenient database tracking
  • Footprint and well spacing match ANSI/SBS standard dimensions
  • Reaction volumes of 5–125 µl (200 µl maximum)
  • Process sampling and testing to ensure that plates are free of DNase, RNase, and human DNA

Compatible Thermal Cyclers

  • Applied Biosystems fast cyclers (9800 fast and Veriti fast)
  • Bio-Rad® C1000™, C1000 Touch, and S1000™
  • Eppendorf Mastercycler series

Compatible Real-Time PCR Detection Systems

  • Applied Biosystems fast instruments (7500 fast and ViiA 7 fast)
  • Bio-Rad® CFX96™, CFX96 Touch™, CFX96 Touch Deep Well™, and CFX Connect™
  • Eppendorf Mastercycler ep realplex

* Hard-Shell plates are covered by one or more of the following U.S. patents or their foreign counterparts owned by Eppendorf AG: U.S. Patent Numbers 7,347,977; 6,340,589; and 6,528,302.



Well/tube format
Well/tube profile
Skirt format
Barcoded PCR plate
Compatible instruments
Applied Biosystems 9800 fast
Applied Biosystems Veriti fast
Applied Biosystems 7500 fast
Applied Biosystems ViiA 7 fast

Bio-Rad® C1000™, C1000 Touch™
Bio-Rad® S1000™
Bio-Rad® CFX96™, CFX96 Touch™
Bio-Rad® CFX96 Touch Deep Well™
Bio-Rad® CFX Connect™

Eppendorf Mastercycler series
Eppendorf Mastercycler ep realplex
Well/tube color