Overview
25 x 20 µl reactions, 100 µl 5x supermix, contains reverse transcriptase, RNase inhibitor, dNTPs, primers, MgCl2, stabilizers; 200 µl no-RT control supermix; 12.5 µl DNase; 150 µl DNase buffer
Available Safety Data Sheets
Safety Data Sheet
Find Certificate of Analysis
Description
Description
Use iScript gDNA Clear cDNA Synthesis Kit for easy and rapid generation of qPCR-ready cDNA free of genomic DNA (gDNA) for accurate gene expression analysis by real-time PCR.
Features and Benefits
- Effective gDNA removal in purified RNA samples
- Proprietary DNase buffer for maintaining RNA integrity during gDNA removal
- Convenient all-in-one, single-tube RT supermix
- Ready-to-use no-RT control supermix for monitoring gDNA contamination
- gDNA-free cDNA ready in 36 min
gDNA-Free cDNA Ready in 36 Minutes
This kit allows you to effectively remove contaminating gDNA and reliably synthesize cDNA with minimal hands-on time in an easy-to-follow protocol, resulting in cDNA free of genomic DNA in 36 minutes.
Packaging Options
| Kit Size | Catalog Number |
| 25 x 20 µl reactions | 1725034 |
| 100 x 20 µl reactions | 1725035 |
| 500 x 20 µl reactions | 1725035BUN |
Supporting Documentation
Download the iScript gDNA Clear cDNA Synthesis Kit Product Insert for detailed information about step-by-step protocols for cDNA synthesis and troubleshooting tips.
Related Products
Related Categories
Specifications
Specifications
Product expiration
(after shipping date)
(after shipping date)
12 months
Storage
Store DNase buffer at 4°C upon receipt. Store all other kit components at –20°C.
Recommended SKUs
Recommended SKUs
Image
1708840
25 x 20 µl reactions, 100 µl 5x supermix, contains reverse transcriptase, RNase inhibitor, dNTPs, primers, MgCl2, stabilizers; 50 reactions of no-RT control supermix