VINEO Brettanomytest PCR Kit

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Overview

PCR detection of Brettanomyces bruxellensis in wine.
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Description

Description

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During the wine manufacturing process, there are two steps of fermentation, alcoholic fermentation then malolactic fermentation. During alcoholic fermentation, yeast transforms sugar into alcohol. Before fermentation, Saccharomyces is present in the grape juice, as well as other yeasts such as Brettanomyces bruxellensis, commonly known as Brett. After alcoholic fermentation, B. bruxellensis can still be present in the matrix.

The presence of B. bruxellensis is an important issue, as it has been shown that its growth is linked to the concentration of 4-ethylphenol, which imparts a strong off-taste, leading to consumer rejection of the wine. To avoid wine spoilage by B. bruxellensis, some preventative actions can be taken such as racking, clarification, and sulfiting. However, if Brett spoilage has already begun, the winemaker must undertake additional remedial steps such as filtration or heat treatment, increasing production cost. If the concentration of ethylphenol is too high, the lot may have to be destroyed.

Rapid Results

Bio-Rad has developed a new tool for the sensitive, selective, and rapid quantification of B. bruxellensis. The VINEO Brettanomytest Kit monitors Brettanomyces risk. The VINEO method is based on real-time PCR technology and entails two simple steps: DNA extraction followed by detection and quantification of the yeast. Current microbiology culture methods can take 7–14 days before results are available, whereas results are available the same day of analysis with the VINEO Kit. The included software automatically provides an easy interpretation of the results.

Ordering

Ordering

items

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3548100
Kit for 96 tests, includes 100 ml lysis solution, 25 ml elution buffer, 100 ml 100x wash solution, 10 ml 1x wash solution, 96 purification columns, 192 collection vials
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3548101
Kit for 96 tests, includes 0.55 ml fluorescent probes, 2 x 2.2 ml amplification mix, 0.5 ml each positive and negative PCR controls
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3600037

Modular thermal cycler platform, includes C1000 Touch Thermal Cycler chassis, CFX96 Deep Well Optical Reaction Module, cables. To be used with CFX Manager Software, Industrial Diagnostic Edition.

Equipment

Equipment

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3600037

Modular thermal cycler platform, includes C1000 Touch Thermal Cycler chassis, CFX96 Deep Well Optical Reaction Module, cables. To be used with CFX Manager Software, Industrial Diagnostic Edition.

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Accessories

Accessories

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ECT2000
Pkg of 1, tool for seating 8- and 12-cap strips on PCR plates or tubes
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2240110
Pkg of 500, screwcap micro test tube, sterilized, with installed O-ring screwcaps
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2240110XTU
Pkg of 500, screwcap micro test tube, sterilized, with installed O-ring screwcaps
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TLS0851
Pkg of 120, thin-wall polypropylene 8-tube strips for PCR and low-volume reactions, white (960 tubes)
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TLS0851XTU
Pkg of 120, thin-wall polypropylene 8–tube strips for PCR and low volume reactions, white, (960 tubes)
TCS0803
Pkg of 120, 8-cap strips for 0.2 ml tubes and plates for PCR and optical reactions, ultraclear
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TCS0803XTU
Pkg of 120, 8-cap strips for 0.2 ml tubes and plates for PCR and optical reactions, ultraclear (960 caps)
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MLL4851
Pkg of 50, white PCR plate, 8 x 6 format, one-component polypropylene, can be cut for fewer samples
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MLL4851XTU
Pkg of 50, white PCR plate, 8 x 6 format, one-component polypropylene, can be cut for fewer samples
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MLL9651
Pkg of 25, white PCR plate, one-component polypropylene, can be cut for fewer samples
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MLL9651XTU
Pkg of 25, white PCR plate, one-component polypropylene, can be cut for fewer samples

Documents

Documents

Number Description Options
FSD_881112_(07-2015)
Instruction Manual, VINEO™ Brettanomytest PCR Kit, Rev 07/2015 (English/Français/Español/Italiano)
FSD_881112_(10-2013)
Instruction Manual, VINEO™ Brettanomytest PCR Kit, Rev 10/2013 (English/Français/Español/Italiano)
6890
VINEO Brettanomytest PCR Test Kit
FSD_881182_(09-2015)
Instruction Manual, VINEO™ Extract DNA Kit, Rev 09/2015 (English/Français/Español/Italiano)
PF_173
CFX Manager™ Software, Industrial Diagnostic Edition, Quick Guide, Rev B
PF_236
Brettanomyces, or Management of a Contaminant Micro-organism, Rev A