Description
Description
During the wine manufacturing process, there are two steps of fermentation, alcoholic fermentation then malolactic fermentation. During alcoholic fermentation, yeast transforms sugar into alcohol. Before fermentation, Saccharomyces is present in the grape juice, as well as other yeasts such as Brettanomyces bruxellensis, commonly known as Brett. After alcoholic fermentation, B. bruxellensis can still be present in the matrix.
The presence of B. bruxellensis is an important issue, as it has been shown that its growth is linked to the concentration of 4-ethylphenol, which imparts a strong off-taste, leading to consumer rejection of the wine. To avoid wine spoilage by B. bruxellensis, some preventative actions can be taken such as racking, clarification, and sulfiting. However, if Brett spoilage has already begun, the winemaker must undertake additional remedial steps such as filtration or heat treatment, increasing production cost. If the concentration of ethylphenol is too high, the lot may have to be destroyed.
Rapid Results
Bio-Rad has developed a new tool for the sensitive, selective, and rapid quantification of B. bruxellensis. The VINEO Brettanomytest Kit monitors Brettanomyces risk. The VINEO method is based on real-time PCR technology and entails two simple steps: DNA extraction followed by detection and quantification of the yeast. Current microbiology culture methods can take 7–14 days before results are available, whereas results are available the same day of analysis with the VINEO Kit. The included software automatically provides an easy interpretation of the results.
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Modular thermal cycler platform, includes C1000 Touch Thermal Cycler chassis, CFX96 Deep Well Optical Reaction Module, cables. To be used with CFX Manager Software, Industrial Diagnostic Edition.
Equipment
Equipment
Modular thermal cycler platform, includes C1000 Touch Thermal Cycler chassis, CFX96 Deep Well Optical Reaction Module, cables. To be used with CFX Manager Software, Industrial Diagnostic Edition.
CFX Manager Software, Industrial Diagnostic Edition
Accessories
Accessories
Pkg of 500, screwcap micro test tube, sterilized, with installed O-ring screwcaps. Note: This item is now available under the new material number 12021141. For future orders please proceed here.
Pkg of 120, thin-wall polypropylene 8-tube strips for PCR and low-volume reactions, white (960 tubes)
Pkg of 120, 8-cap strips for 0.2 ml tubes and plates for PCR and optical reactions, ultraclear