SsoFast EvaGreen Supermixes

Image
ssofast-evagreen-supermix-detail.jpg

Overview

SsoFast EvaGreen supermixes employ our patented Sso7d fusion enzyme technology to provide fast real-time PCR results, robust target discrimination, and high reproducibility.

Read full description Ordering Information

Description

Description

NEW PRODUCT
SsoAdvanced Universal Supermixes

Affordable, superior performance alternative to SsoFast Supermixes

  • Guaranteed performance under all conditions
  • Use on any real-time PCR instrument without adjusting ROX concentration
SsoAdvanced Universal Supermixes

SsoFast EvaGreen Supermix utilizes our patented Sso7d fusion protein technology* for high performance in a wide range of qPCR applications. The dsDNA-binding protein Sso7d stabilizes the polymerase-template complex, increasing processivity and reducing reaction times compared with conventional DNA polymerases without affecting PCR sensitivity, efficiency, or reproducibility. EvaGreen formulation provides increased fluorescence compared to SYBR Green.

Key Features and Benefits

  • Fast qPCR results and high performance — instant activation and rapid polymerization kinetics generate exceptional qPCR results in less than 30 min
  • Minimal PCR inhibition — increased resistance to PCR inhibitors ensures maximum efficiency, sensitivity, and reproducibility
  • Single-copy detection — provides high sensitivity with amplification and detection from a single copy of a target gene
  • Robust discrimination and reproducibility — achieves efficient discrimination and reliable quantification from 1.33-fold serial dilutions of template

Applications and Uses

  • Dye-based qPCR detection
  • Gene expression analysis
  • Genotyping studies

Related Products

Select the best real-time PCR supermix for your application:

  • Bio-Rad’s Universal Supermixes can be used in all real-time PCR systems — both ROX-dependent and ROX-independent instruments
  • SsoAdvanced Universal Supermixes feature our patented Sso7d fusion polymerase

Key Features of Bio-Rad Real-Time PCR Supermixes

SsoFast
Supermixes

SsoAdvanced Universal Supermixes

Sso7d fusion protein technology

Yes

Yes

Sensitive detection of low-level targets

Efficiency with difficult target sequences

Specificity

Tolerance to PCR inhibitors

Broad range of reaction conditions

Fast cycling

Instrument compatibility

Select instruments

Any instrument

 

Related Categories

 

* U.S. patent 7,560,260.

Specifications

Specifications

Product expiration (after shipping date)
12 months
Storage at 4°C (after thawing)
Up to 6 months
Customer guarantee (minimum usage time) at –20°C
12 months

Ordering

Ordering

items

Use the filters below to refine results!

Image
172-5200_tn.jpg
1725200
2 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, EvaGreen dye, stabilizers, for 200 x 20 µl reactions
Image
172-5201_tn.jpg
1725201
5 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, EvaGreen dye, stabilizers, for 500 x 20 µl reactions
Image
172-5202_tn.jpg
1725202
10 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, EvaGreen dye, stabilizers, for 1,000 x 20 µl reactions
Image
172-5203_tn.jpg
1725203
20 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, EvaGreen dye, stabilizers, for 2,000 x 20 µl reactions
Image
172-5204_tn.jpg
1725204
25 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, EvaGreen dye, stabilizers, for 2,500 x 20 µl reactions
Image
172-5205_tn.jpg
1725205
50 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, EvaGreen dye, stabilizers, for 5,000 x 20 µl reactions

Documents

Documents

Number Description Options
10014647
Instruction Manual, SsoFast EvaGreen Supermix, Rev D
5936
SsoFast EvaGreen Supermix Stability for Automated qPCR, Rev A
6252
Reagent Comparison Guide for Real-Time PCR Brochure, Rev C
[ Add to Cart (Free) ]
7178
Amplification: Real-Time PCR Reagents and Kits Brochure
6090
Amplification Reagents and Plastics Brochure
[ Add to Cart (Free) ]
6763
Gene Expression Real-Time PCR Workflow Flier
[ Add to Cart (Free) ]