SsoFast EvaGreen Supermixes

SsoFast EvaGreen Supermixes

SsoFast EvaGreen supermixes employ our patented Sso7d fusion enzyme technology to provide fast real-time PCR results, robust target discrimination, and high reproducibility.

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Overview

Affordable, superior performance alternative to SsoFast Supermixes

  • Guaranteed performance under all conditions
  • Use on any real-time PCR instrument without adjusting ROX concentration

SsoFast EvaGreen Supermix utilizes our patented Sso7d fusion protein technology* for high performance in a wide range of qPCR applications. The dsDNA-binding protein Sso7d stabilizes the polymerase-template complex, increasing processivity and reducing reaction times compared with conventional DNA polymerases without affecting PCR sensitivity, efficiency, or reproducibility. EvaGreen formulation provides increased fluorescence compared to SYBR Green.

Key Features and Benefits

  • Fast qPCR results and high performance — instant activation and rapid polymerization kinetics generate exceptional qPCR results in less than 30 min
  • Minimal PCR inhibition — increased resistance to PCR inhibitors ensures maximum efficiency, sensitivity, and reproducibility
  • Single-copy detection — provides high sensitivity with amplification and detection from a single copy of a target gene
  • Robust discrimination and reproducibility — achieves efficient discrimination and reliable quantification from 1.33-fold serial dilutions of template

Applications and Uses

  • Dye-based qPCR detection
  • Gene expression analysis
  • Genotyping studies

Related Products

Select the best real-time PCR supermix for your application:

  • Bio-Rad’s Universal Supermixes can be used in all real-time PCR systems — both ROX-dependent and ROX-independent instruments
  • SsoAdvanced Universal Supermixes feature our patented Sso7d fusion polymerase
Key Features of Bio-Rad Real-Time PCR Supermixes SsoFast Supermixes SsoAdvanced Universal Supermixes
Sso7d fusion protein technology Yes Yes
Sensitive detection of low-level targets
Efficiency with difficult target sequences
Specificity
Tolerance to PCR inhibitors
Broad range of reaction conditions
Fast cycling
Instrument compatibility Select instruments Any instrument

Related Categories

* U.S. patent 7,560,260.

Specifications

Product expiration (after shipping date)
12 months
Storage at 4°C (after thawing)
Up to 6 months
Customer guarantee (minimum usage time) at –20°C
12 months

Ordering

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172-5200_tn.jpg
(Discontinued) SsoFast™ EvaGreen® Supermix, 200 x 20 µl rxns, 2 ml (2 x 1 ml)
1725200
2 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, EvaGreen dye, stabilizers, for 200 x 20 µl reactions
172-5201_tn.jpg
(Discontinued) SsoFast™ EvaGreen® Supermix, 500 x 20 µl rxns, 5 ml (5 x 1 ml)
1725201
5 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, EvaGreen dye, stabilizers, for 500 x 20 µl reactions
172-5202_tn.jpg
(Discontinued) SsoFast™ EvaGreen® Supermix, 1,000 x 20 µl rxns, 10 ml (10 x 1 ml)
1725202
10 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, EvaGreen dye, stabilizers, for 1,000 x 20 µl reactions
172-5203_tn.jpg
(Discontinued) SsoFast™ EvaGreen® Supermix, 2,000 x 20 µl rxns, 20 ml (1 x 20 ml)
1725203
20 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, EvaGreen dye, stabilizers, for 2,000 x 20 µl reactions
172-5204_tn.jpg
(Discontinued) SsoFast™ EvaGreen® Supermix, 2,500 x 20 µl rxns, 25 ml (5 x 5 ml)
1725204
25 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, EvaGreen dye, stabilizers, for 2,500 x 20 µl reactions
172-5205_tn.jpg
(Discontinued) SsoFast™ EvaGreen® Supermix, 5,000 x 20 µl rxns, 50 ml (10 x 5 ml)
1725205
50 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, EvaGreen dye, stabilizers, for 5,000 x 20 µl reactions

Documents

Number Description Options
10014647
Instruction Manual, SsoFast EvaGreen Supermix, Rev D
5936
SsoFast EvaGreen Supermix Stability for Automated qPCR, Rev A
6252
Reagent Comparison Guide for Real-Time PCR Brochure, Rev C
[ Add to Cart (Free) ]
7178
Amplification: Real-Time PCR Reagents and Kits Brochure
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6090
Amplification Reagents and Plastics Brochure
6763
Gene Expression Real-Time PCR Workflow Flier
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