Rotofor® and Mini Rotofor Cells

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Overview

The Rotofor® family of products has been discontinued. We will continue to provide support for Rotofor owners until March, 2020.
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Description

Description

Discontinued Product
The Rotofor® family of products has been discontinued. We will continue to provide support for Rotofor owners until March, 2020.

 

The Rotofor and Mini Rotofor cells use liquid-phase IEF to separate proteins into 20 fractions in free solution. As an initial purification step, these cells concentrate proteins and eliminate those outside of a pI range of interest. As a final purification step, they eliminate protein contaminants that may be difficult to remove by other means.

Because it can process up to gram quantities of protein and effectively concentrates proteins into discrete zones based on pI, the Rotofor is a proven and effective method for the enrichment of low-abundance proteins. In a single run, the Rotofor cell concentrates samples up to 20-fold; selected fractions may also be pooled and refractionated on either the Mini Rotofor cell or the MicroRotofor™ cell to yield even greater purification. In 2-D electrophoresis applications, protein fractionation by pI improves downstream sample loading and separation on narrow- and micro-range IPG strips by eliminating proteins outside the pI region of interest.

Accessory equipment required to run the Rotofor and Mini Rotofor cells includes a 3,000 V power supply such as the PowerPac™ HV power supply, a recirculating water chiller, and house vacuum. Bio-Lyte® ampholytes are required to form the internal pH gradient.

Related Products

Different configurations of the Rotofor system are available to accommodate different sample loads. The Rotofor, Mini Rotofor, and MicroRotofor cells all share the following features:

  • Interchangeable cylindrical focusing chambers, which enable refractionation of selected fractions for even greater purification
  • Maintenance of biological activity
  • Customizable pH gradients — wide or narrow linear pH gradients can be generated using Bio-Lyte ampholytes
  • Focusing and enrichment of a protein of interest into 1 or 2 fractions in a single step

 

  Rotofor Cell Mini Rotofor Cell MicroRotofor Cell
Number of fractions 20 20 10
Sample volume 35–60 ml 18 ml 2.5 ml
Fraction volume 1.75–3 ml 0.7 ml 200–250 µl
Sample load Milligram to gram Microgram to milligram Up to to 1 mg

Ordering

Ordering

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(Discontinued) Rotofor Starter Kit
1702910
Kit includes 10 ml Bio-Lyte ampholytes, 60 ml syringe, colored protein sample, 2 vent buttons, 2 ion exchange membranes, hydrated, for use with Rotofor products
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1702919
1 ml, colored proteins, for use with Rotofor systems
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1702950
100/120 V, preparative IEF cell with 60 ml focusing chamber, sample volume 35–60 ml, includes starter kit
170-2951_tn.jpg
1702951
220/220 V, preparative IEF cell with 60 ml focusing chamber, sample volume 35–60 ml, includes starter kit
170-2986_tn.jpg
1702986
100/120 V, preparative IEF cell system, sample volume 35–60 ml, includes 60 ml and 18 ml focusing chambers, starter kit
170-2987_tn.jpg
1702987
220/240 V, preparative IEF cell system, sample volume 35–60 ml, includes 60 ml and 18 ml focusing chambers, starter kit
170-2988_tn.jpg
(Discontinued) Mini Rotofor Cell
1702988
100/120 V, preparative IEF cell, sample volume 18 ml, includes 18 ml focusing chamber, starter kit
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(Discontinued) Mini Rotofor Cell
1702989
220/240 V, preparative IEF cell, sample volume 18 ml, includes 18 ml focusing chamber, starter kit
1702906
220/240 V, preparative IEF cell system and power supply (#164-5056), sample volume 35–60 ml, includes 60 ml and 18 ml focusing chambers, starter kit
1702914
100/120 V, preparative IEF cell system and PowerPac™ HV power supply (#164-5056), sample volume 35–60 ml, includes 60 ml and 18 ml focusing chambers, starter kit

Documents

Documents

Number Description Options
3131
The Rotofor System As a Prefractionation Device Used Prior to Electrophoresis, Rev A