Bio-Rad’s ASCA EIA kits are intended for the semi-quantitative and qualitative determination of IgA or IgG antibodies to cell wall proteoglycans of Saccharomyces cerevisiae in human serum. ASCA assays aid in the diagnosis of Crohn’s disease, a chronic inflammatory disorder of the gastrointestinal tract. Saccharomyces cerevisiae is commonly known as baker’s yeast or brewer’s yeast. A positive anti-Saccharomyces cerevisiae antibodies (ASCA) test and a negative perinuclear anti-neutrophil cytoplasmic antibody (p-ANCA) test help to differentiate Crohn’s disease from ulcerative colitis, the other form of inflammatory bowel disease.
Bio-Rad’s Autoimmune ASCA EIA kits feature common protocols and common reagents. All reagents and reagent bottle caps are color-coded for ease of use. Test operation is further streamlined with breakaway wells and easy automation.
- Sample dilution: 1:101
- Quantitative and qualitative calibration option
- Incubation time: 30/30/30 minutes
- Incubation temperature: room temperature (20–26°C)
- Cut-off: 15 U/mL
- Conjugate: HRP
- Substrate: TMB
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