ddPCR Cell and Gene Therapy Assays

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Overview

For a range of Cell and Gene Therapy applications including Viral Titer and Vector Copy Number measurements.

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Description

Description

Vector Backbone Assays

Browse a list of ddPCR Expert Design Assays for common Viral Vector Backbone or Plasmid Elements.

Vector Backbone Assays

Bio-Rad’s ddPCR Cell and Gene Therapy (CGT) Assays are custom ddPCR-optimized assays generated by the Cell and Gene Therapy Design Engine for your target Gene of Interest. These assays offer the ease and accuracy of ddPCR technology and come in multiple fluorophores to enable multiplexed Cell and Gene Therapy applications, including Viral Titer and Vector Copy Number measurements. Use the ddPCR CGT Assays individually or multiplex them with the Vector Backbone Assays to precisely measure the concentration of the therapeutic gene in your sample.

Features and Benefits

  • Quick assay design through the Cell and Gene Therapy Design Engine, saving you valuable time to focus on important experiments
  • Robust assay design, optimized specifically for ddPCR technology
  • Six probe fluorophore options to enable multiplexing
  • Vector Backbone Assays available for multiplexing
  • In-silico multiplexing checks (wet-lab validation recommended)
  • Certificate of Analysis provided with each ddPCR CGT Assay order
  • Assay sequence provided with select catalog numbers

Applications and Uses

  • Viral Vector Genome Titer Measurement
  • Vector Copy Number Determination
  • Gene Therapy Development and Manufacturing
  • CAR-T Cell Therapy Development and Manufacturing

Sample Data

2-D fluorescence amplitude plotBar graphs of the average concentration of AAV ITR-2 and GOI targets% CV plot showing the high precision in viral titer measurements

ddPCR Cell and Gene Therapy Assays provide superior resolution and precision. A, 2-D fluorescence amplitude plot shows six replicate wells of an AAV sample (virus dilution 2) assayed on the QX200 AutoDG ddPCR System with the AAV ITR-2 Assay in FAM (ddPCR EXD Assay: dEXD15274642), duplexed with a custom Gene of Interest (GOI) Assay in HEX (ddPCR CGT Assay), and MspI as the compatible restriction enzyme. The black cluster on the plot represents negative droplets, the blue cluster represents droplets that are positive for AAV ITR-2 only, the green cluster represents droplets that are positive for GOI only, and the orange cluster represents droplets that are positive for both AAV ITR-2 and GOI targets. B, Concentration plot shows the average AAV ITR-2 and GOI target concentrations for three AAV sample dilutions and No-template Control (NTC). Note the consistent AAV ITR-2:GOI ratio, which can be used as an indicator of AAV packaging efficiency. C, % CV plot shows the high precision in viral titer measurements obtained for each AAV sample dilution using the AAV ITR-2 x GOI duplex ddPCR assay set in this example.

Packaging Options

ddPCR Cell and Gene Therapy are provided in a 20X ready-to-use primer-probe mix, available in six fluorophore choices, optimized for use with ddPCR Supermix for Probes (No dUTP) or ddPCR Multiplex Supermix.

Products Catalog
ddPCR CGT Assay (FAM), 200 x 20 μl reactions 12014589
ddPCR CGT Assay (HEX), 200 x 20 μl reactions 12014590
ddPCR CGT Assay (FAM + HEX), 200 x 20 μl reactions 12014641
ddPCR CGT Assay (Cy5), 200 x 20 μl reactions 12014642
ddPCR CGT Assay (Cy5.5), 200 x 20 μl reactions 12014643
ddPCR CGT Assay (Cy5 + Cy5.5), 200 x 20 μl reactions 12014644
ddPCR CGT Assay (FAM), 1,000 x 20 μl reactions 12014645
ddPCR CGT Assay (HEX), 1,000 x 20 μl reactions 12014646
ddPCR CGT Assay (FAM + HEX), 1,000 x 20 μl reactions 12014647
ddPCR CGT Assay (Cy5), 1,000 x 20 μl reactions 12014648
ddPCR CGT Assay (Cy5.5), 1,000 x 20 μl reactions 12014649
ddPCR CGT Assay (Cy5 + Cy5.5), 1,000 x 20 μl reactions 12014650
ddPCR CGT Assay (FAM), 1,000 x 20 μl reactions* 12014651
ddPCR CGT Assay (HEX), 1,000 x 20 μl reactions* 12014652
ddPCR CGT Assay (FAM + HEX), 1,000 x 20 μl reactions* 12014653
ddPCR CGT Assay (Cy5), 1,000 x 20 μl reactions* 12014654
ddPCR CGT Assay (Cy5.5), 1,000 x 20 μl reactions* 12014655
ddPCR CGT Assay (Cy5 + Cy5.5), 1,000 x 20 μl reactions* 12014656
* Primer and probe sequences provided.  

Related Categories

Resources

Documents

Documents

Number Description Options
10000132898
ddPCR Cell and Gene Therapy Assays
7320
Transitioning Your Assay from Quantitative PCR to Droplet Digital PCR
7407
Measuring Adeno-Associated Virus (AAV) Vector Genome Titer Using Droplet Digital PCR Protocol
7425
ddPCR Assays for Adeno-Associated Virus (AAV) Vector Genome Titer Flier