Practical Steps to Improve qPCR Data Quality
The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines were first introduced in 2009 to standardize the design, execution, and transparent reporting of research that used quantitative PCR (qPCR). In 2025, these guidelines were comprehensively updated and republished as MIQE 2.0, reflecting the advances in qPCR technologies and applications feedback on the original text.
This webinar will provide attendees with a practical overview of MIQE 2.0, highlighting considerations for generating accurate, reproducible data to enable experimental findings to deliver greater impact. Topics will include rigorous documentation of sample handling, nucleic acid extraction, and assay validation, methods for calculating assay efficiency, and the critical role of instrument calibration. All content is presented in an accessible format for immediate use and future reference.
Designed for both newcomers to qPCR and experienced researchers, this session emphasizes real-world application and appropriate considerations rather than rigid dictates (mandates), ensuring methodological stringency without unnecessary burden.
Learning Objectives:
- Review key recommendations from the MIQE 2.0 publication
- Learn how to convert quantification cycle (Cq) values into efficiency-corrected target quantities with statistically justified prediction intervals
- Understand how detection limits and dynamic ranges can be influenced by preanalytical considerations
- Clarify best practices for reference gene normalization, quality control, and the use of appropriate positive and negative controls
Speaker
Jim Huggett
Director of Biological Metrology at the UK National Measurement Laboratory (NML)
Jim Huggett is a molecular biologist and biometrologist with international expertise in biological measurement science. He serves as Director of Biological Metrology at the UK National Measurement Laboratory (NML), providing strategic leadership across a multidisciplinary life sciences portfolio.
Over a career spanning more than 20 years, his work has been influenced by PCR, with a particular interest in ensuring its accuracy in molecular measurements to underpin confidence and ultimately impact of everyday research. He is widely recognized for his contributions to measurement best practices, including the MIQE guidelines, helping researchers produce robust, comparable, and reliable results.
His work bridges academic research, measurement standards, and their real-world application, supporting the generation of high-quality molecular data.
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Checklist on MIQE 2.0 Guidelines for Real-Time PCR
Ensure your qPCR experiments meet the latest MIQE 2.0 recommendations. This practical checklist covers best practices across the qPCR workflow to help you identify gaps and generate reproducible, publication-ready data.
