AEX Mixed-Mode Chromatography Resin for High Selectivity and Recovery
This webinar introduces the features of Nuvia aPrime4A , a hydrophobic AEX chromatography resin , that have been optimized to facilitate selective and reversible binding of molecules for high purity and recovery over traditional chromatography resins.
CHT Column Packing is Easier Than You Think
This webcast presents best practices and guidelines for packing CHT Ceramic Hydroxyapatite Media at process scale. CHT, is an easy-to-use mixed-mode chromatography media. It offers cation exchange and affinity interactions. Unlike other chromatography media, CHT is both the ligand and the support matrix. It is used for the purification of numerous types of biomolecules and is considered the gold standard for aggregate removal.
Nuvia HP-Q- A New High Performance AEX Resin
Nuvia HP-Q is our newest strong anion exchange resin. It provides both high binding capacity and high recovery, even at high flow rates. A rigid bead with large pores and optimized surface extenders, this resin was designed for large biomolecule purification. Dr. Jiali Liao will present case studies and design features to meet manufacturing demands.
CHT Ceramic Hydroxyapatite Resin Packing Guidelines
This video demonstrates proper technique for packing and unpacking CHT Ceramic Hydroxyapatite Resin, provides practical media specifications for optimal hardware and process conditions for best results and long media life.
Ceramic Hydroxyapatite Chromatography Media — How to Start
Ceramic Hydroxyapatite XT, is a calcium affinity cation exchange chromatography media. It is used for the purification of numerous types of biomolecules and provides single-step clearance of impurities and aggregates for monoclonal antibody purification. CHT XT is an easy-to-use mixed-mode chromatography media. Unlike other chromatography media it is both the ligand and the support matrix. In this webinar our expert, Dr. Mark Snyder, will discuss the application use of CHT XT at process-scale and provide guidelines on where to start when selecting a hydroxyapatite media for your purification.
Challenges and Opportunities for Improved Chromatography Resins
Professor Giorgio Carta presents a summary of Challenges and Opportunities for Improved Resins for the Chromatographic Processing of Biopharmaceuticals presentation at BPI. He discussed an overview of recent advances in the development of resins for protein purification and in understanding protein-surface interactions that are responsible for separation. He presented that new large-pore resins with small particle size based on a hydrophilic backbone can prevent these phenomena resulting in more effective separation.
Packing CHT Resin in an Open Process Column
These guidelines for handling and packing CHT Ceramic Hydroxyapatite Resin in an open process column are recommended for best results with CHT Resins.
CHT Ceramic Hydoxyapatite: Slurry Transfer and Packing in an Open Process-Scale Column
This video demonstrates guidelines for packing CHT in an open process-scale columns. The video details slurry transfer by hose, container or creating the slurry in the open process column. These guidelines for handling and packing are recommended for all CHT users to ensure reproducible and high-quality results.
CHT Ceramic Hydoxyapatite: Slurry Transfer and Packing in a Closed Process-Scale Column
This video demonstrates guidelines for packing CHT in a closed process-scale columns by slurry valve transfer. Larger process-scale columns are typically closed systems where the media slurry must be introduced through slurry valves. The slurry is injected through these valves into the column. These guidelines for handling and packing are recommended for all CHT users to ensure reproducible and high-quality results
Effective Processes for Virus Purification
This presentation is focused on
- Understanding the challenges in virus/VLP purification
- Choosing the appropriate (or right) chromatography media for the production virus particles
- Developing chromatography methods for optimal binding selectivity and recovery of virus particles
Purification Platform for IgMs
This presentation is focused on
- Understanding the challenges of IgM purification
- Designing a scalable non-affinity based purification platform for manufacture of diagnostic IgMs
Nuvia Q and Nuvia S Ultra-High Capacity Ion Exchange Resins
Bio-Rad's Nuvia Resins are a family of best-in-class ion exchangers built with exceptionally high capacities and excellent purification capabilities, providing process developers with greater flexibility and higher productivity.
CHT Ceramic Hydroxyapatite for Commercial Purification
Bio-Rad's CHT Resins are innovative mixed-mode media that offer unique separation properties and unparalleled selectivity and resolution for a range of molecules. These resins can be used at any stage in a purification process, from initial capture to final polishing.
A Scalable Immobilized Metal Affinity Chromatography Media for Process Purification
Downstream process purification of proteins requires a resin with optimized bead size for ideal pressure/flow properties and decent dynamic binding capacity DBC that provides production efficiencies and good process economics. Nuvia IMAC – provides the mechanical strength, pore structures, ligand density, and particle size distribution required for an operation run at 300 cm/hr with a DBC of 40 mg/ml at 2 bar column backpressure. The resin is also compatible with reagents typically employed for histidine-tagged protein purification. Nuvia IMAC Resins overcome the challenges associated with process purification of proteins using metal affinity chromatography.
What is CHT Ceramic Hydroxyapatite Resin?
CHT Resin is a versatile chromatography resin for the separation of biological molecules including antibodies, enzymes, nucleic acids, and membrane proteins. This mixed-mode resin contains both ion exchange and metal affinity sites, providing unique selectivities.