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|RNA Sample Preparation
for COVID-19 Detection
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Chelex 100 Resin for RNA Sample Preparation
Discover how Chelex 100 Resin compares to a conventional RNA extraction kit.Learn More
Chelex* Chelating Resins are produced by Bio-Rad and widely used for a range of applications. Chelex 100 Resin**, and related InstaGene Matrix**, have long been trusted for the preparation of nucleic acids from many sample types including forensic samples, blood, tissues, cell cultures, parasites, insects, and bacteria.
Streamline PCR or LAMP Sample Preparation with Chelex 100 Resin
Molecular detection methods are instrumental; from criminal forensics to food and clinical pathogen detection. Nucleic acids, such as DNA or RNA, collected from these samples need to be first isolated before downstream processing and analysis by PCR, ddPCR, or LAMP. This isolation is often performed with commercially available nucleic acid extraction kits designed for specific applications. Such kits tend to be costly and are used even in applications where highly purified nucleic acids are not required. In addition, these kits can generate a significant amount of chemical and plastic waste.
Chelex 100 Resin is an alternative to extraction kits and enables rapid DNA or RNA sample preparation at a fraction of the cost, while generating less waste.
Advantages of Chelex 100 Resin for Nucleic Acid Sample Preparation
- Fast, simple workflow
- Significant cost-savings over nucleic acid extraction kits
- Long shelf life (3 years***)
- Trusted manufacturer
- Scalable for high-throughput screening
Chelex 100 Resin Inhibits Nuclease Activity
Chelex 100 Resin is designed to strongly bind divalent metal ions such as Mg2+ that are required for the activity of metallo-nucleases. The addition of Chelex 100 Resin effectively inhibits nuclease activity in complex samples and stabilizes samples for downstream PCR applications. Simultaneously, Chelex 100 Resin efficiently removes PCR inhibitors. Thus, Chelex 100 Resin can be utilized in place of expensive DNA or RNA extraction kits.
Sample Preparation Workflow
Chelex 100 Resin enables DNA or RNA preparations from complex samples without DNA or RNA extraction kits. A fast, simple four-step workflow is typically sufficient for the preparation of nucleic acids from a wide variety of sample types. Additional reagents can easily be combined with Chelex 100 Resin if required for the specific sample/application.
RNA Sample Preparation for COVID-19 Detection with Chelex 100 Resin
The importance of molecular diagnostics to inform public health decisions was evident during the COVID-19 pandemic. Driven by shortages and supply-chain disruptions, many laboratories and organizations discovered that Chelex 100 Resin can be utilized as an alternative to RNA extraction kits.
Learn how laboratories have validated the use of Chelex 100 Resin to prepare samples for viral RNA detection in the following featured articles and discover a simple protocol utilizing Chelex 100 Resin or InstaGene Matrix as an effective alternative to an extraction kit (Bulletin 3318).
Featured Articles: Application of Chelex 100 Resin for SARS-CoV-2 RNA Sample Preparation
Sensitive extraction-free SARS-CoV-2 RNA virus detection using a chelating resin
Guan B et al. iScience. 2021 Sep; 24: 102960. DOI: 10.1016/j.isci.2021.102960
A simple direct RT-LAMP SARS-CoV-2 saliva diagnostic.
Flynn MJ et al. medRxiv 2020.11.19.20234948; DOI: 10.1101/2020.11.19.20234948
A point-of-care SARS-CoV-2 test based on reverse transcription loop-mediated isothermal amplification without RNA extraction with diagnostic performance same as RT-PCR
Odiwuor N et al. Analytica Chimica Acta 2022 Feb; 1200. DOI: 10.1016/j.aca.2022.339590
A simple method for SARS-CoV-2 detection by rRT-PCR without the use of a commercial RNA extraction kit. Ulloa S et al. J Virol Methods. 2020 Nov;285:113960. DOI: 10.1016/j.jviromet.2020.113960.
Chelex 100 Resin (Molecular Biology Grade) allows the optimization of the Chelex 100 Resin suspension to fit your application; it is particularly suited for processing large amounts of samples.
InstaGene Matrix is a ready-to-use, optimized Chelex 100 Resin suspension that is quality controlled for lot-to-lot reproducibility. It is offered as a convenient, finished formula for preparing routine samples.
Chelex 100 Resin (Molecular Biology Grade)
50 g, molecular biology grade cation exchange resin, sodium form, 1% crosslinkage, 200–400 dry mesh size, 75–150 µm wet bead size, ~3,500 MW limit.Order Now
20 ml, Chelex-based resin for PCR-ready DNA purification, for 100 extractionsOrder Now
Chelex 100 Resin has long been used for fast, easy DNA preparation from many sample types, including blood, tissues, parasites, insects and bacteria.
For the use of Chelex 100 Resin in Forensic and Population Genetic Analyses, view our publication list.
Kolia-Diafouka P et al. Optimized Lysis-Extraction Method Combined with IS6110-Amplification for Detection of Mycobacterium tuberculosis in Paucibacillary Sputum Specimens.
Front Microbiol. 2018 Sep 25;9:2224. DOI: 10.3389/fmicb.2018.02224
Lee RA et al. Ultrasensitive CRISPR-based diagnostic for field-applicable detection of Plasmodium species in symptomatic and asymptomatic malaria Proc Natl Acad Sci U S A.
2020 Oct 13;117(41):25722-25731. DOI: 10.1073/pnas.2010196117
Lienhard A and Schäffer S. Extracting the invisible: obtaining high quality DNA is a challenging task in small arthropods.
PeerJ. 2019 Apr 12;7:e6753. DOI: 10.7717/peerj.6753
Mayran C et al. Rapid diagnostic test for Hepatitis B virus viral load based on recombinase polymerase amplification combined with a lateral flow read-out
Diagnostics 2022 Mar; 12(3): 621. DOI: 10.3390/diagnostics12030621
Musapa M et al. A simple Chelex protocol for DNA extraction from Anopheles spp.
J Vis Exp. 2013 Jan 9;(71):3281. DOI: 10.3791/3281
Mygind T et al. Determination of PCR efficiency in Chelex 100-purified clinical samples and comparison of real-time quantitative PCR and conventional PCR for detection of Chlamydia pneumoniae.
BMC Microbiol. 2002 Jul 9;2:17. DOI: 10.1186/1471-2180-2-17
Nouemssi SB et al. Rapid and Efficient Colony-PCR for High Throughput Screening of Genetically Transformed Chlamydomonas reinhardtii.
Life (Basel). 2020 Sep 10;10(9):186. DOI: 10.3390/life10090186
Simon N et al. Optimization of Chelex 100 resin-based extraction of genomic DNA from dried blood spots.
Biol Methods Protoc. 2020 May 2;5(1):bpaa009. DOI: 10.1093/biomethods/bpaa009
Tomasek O et al. Comparison of three template preparation methods for routine detection of beak and feather disease virus and avian polyomavirus with single and nested polymerase chain reaction in clinical specimens.
Avian Pathol. 2008 Apr;37(2):145-9. DOI: 10.1080/03079450801902047
Tomazic ML et al. Use of Chelex-100 for the molecular diagnosis of five animal pathogens
FAVE Sección Ciencias Veterinarias 2021 Apr; 26-33. DOI: 10.14409/favecv.v20i1.9724
Walsh PS et al. Chelex 100 as a Medium for Simple Extraction of DNA for PCR-Based Typing from
Biotechniques 2013 Mar;54(3):134-9. DOI: 10.2144/000114018
Yarimizu K et al. Development of an absolute quantification method for ribosomal. RNA gene copy numbers per eukaryotic single cell by digital PCR.
Harmful Algae. 2021 Mar;103:102008. doi: 10.1016/j.hal.2021.102008.
The application of Chelex 100 Resin for RNA sample preparation has shown rapid adoption in recent years as an optimal and cost-saving alternative to RNA extraction kits.
Discover our simple protocol utilizing Chelex 100 Resin or InstaGene Matrix as an effective alternative to an RNA extraction kit here.
Bauer M & Patzelt D. A method for simultaneous RNA and DNA isolation from dried blood and semen stains.
Forensic Sci Int. 2003 Sep 9:136(1-3):76-8. doi: 10.1016/s0379-0738(03)00219-6.
Coombs NJ et al. Optimisation of DNA and RNA extraction from archival formalin-fixed tissue.
Nucleic Acids Res. 1999 27(16): e1227:e12. DOl: 10.1093/nar/27.16.e12
Elnagar A et al. Optimizing Release of Nucleic Acids of African Swine Fever Virus and Influenza A
Virus from FTA Cards.
Int J Mol Sci. 2021 Nov 29; 22(23):12915. doi: 10.3390/ims222312915.
Flynn MJ et al. A simple direct RT-LAMP SARS-CoV-2 saliva diagnostic.
medRxiv 2020.11.19.20234948; DOl: 10.1101/2020.11.19.20234948
Guan B et al. Sensitive extraction-free SARS-CoV-2 RNA virus detection using a chelating resin.
Science. 2021 Sep; 24: 102960. DOl: 10.1016/j.isci.2021.102960
Hale AD et al. Comparison of four RNA extraction methods for the detection of small round structured viruses in faecal specimens.
J Virol Methods. 1996 Apr 5;57(2):195-201. DOl: 10.1016/0166-0934(95)01966-9
Howson E et al. Preliminary optimisation of a simplified sample preparation method to permit direct detection of SARS-CoV-2 within saliva samples using reverse-transcription loop-mediated isothermal amplification (RT-LAMP).
J Virol Methods. 2020 Nov; 114048. DOl: 10.1016/j.jviromet.2020.114048
Lai MY et al. RNA purification-free detection of SARS-CoV-2 using reverse
transcription loop-mediated isothermal amplification (RT-LAMP).
Trop Med Health. 2022 Jan 4;50(1):2. doi: 10.1186/s41182-021-00396-y
Odiwuor N et al. A point-of-care SARS-CoV-2 test based on reverse transcription loop-mediated isothermal
amplification without RNA extraction with diagnostic performance same as RT-PCR
Analytica Chimica Acta 2022 Feb; 1200. DOl: 10.1016/.aca.2022.339590
Ulloa S et al. A simple method for SARS-CoV-2 detection by rRT-PCR without the use of a commercial RNA extraction kit.
J Virol Methods. 2020 Nov; 285:113960. DOl: 10.1016/jjviromet.2020.113960.
Thatcher SA. DNA/RNA Preparation for Molecular Detection.
Clinical Chemistry, Volume 61, Issue 1, 1 January 2015, Pages 89-99. DOl: 10.1373/clinchem.2014.221374
Yaren O et al. Ultra-rapid detection of SARS-CoV-2 in public workspace environments
PLOS One. 2021 Feb; 0240524. DOl: 10.1371/journal.pone.0240524