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PTC Tempo Thermal Cycler

PTC Tempo Thermal Cycler

Choose the PTC Tempo Thermal Cycler, which offers the thermal accuracy and uniformity you can trust to get the consistent performance you need in PCR.

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Overview

New Version Available!

PTC Tempo Software 3.0.0.8 is now available for all PTC Tempo models. This update incorporates all the PTC Tempo Thermal Cycler models, connectivity to Bio-Rad’s BR.io app to manage PCR protocol files and view run status, and the ability to integrate with laboratory automation systems using the PTC Tempo Automation API. Go to bio-rad.com/PCRUpdates to upgrade your PTC Tempo thermal cycler's software and firmware. Follow directions within the 10000160470 Release Notes to upgrade your PTC Tempo today.

OPUS-TOUR PTC Tempo System Tour

The PTC Tempo Thermal Cycler represents the next generation of conventional PCR thermal cyclers from Bio-Rad. Engineered to maintain thermal performance that delivers accurate results, the PTC Tempo Thermal Cycler offers the flexibility to scale with your laboratory. The new design features a refreshed, intuitive user interface and flexible connectivity options for streamlining protocol management. A motorized lid enables automation, taking your PCR to the next level with modern instrument control.

The PTC Tempo Thermal Cycler is available in multiple fixed-block configurations: PTC Tempo 96 Thermal Cycler for most general PCR applications and PTC Tempo Deepwell Thermal Cycler with larger volumes up to 125 µl.

Features and Benefits

  • Programmable thermal gradient — for optimization of annealing temperatures
  • Large touch screen — for streamlined protocol programming and editing
  • Heated lid — for use with various vessels and sealer types
  • Expanded connectivity — Wi-Fi, ethernet, and USB
  • Automated lid — for integration with automation robotics
  • Cloud connectivity — create and send protocols from your BR.io cloud platform
  • Network storage drive access — for excellent data management

Applications and Uses

  • Amplification/PCR
  • Cloning
  • Cycle sequencing
  • Gene expression studies
  • Mutagenesis

Thermal Gradient

Thermal Gradient

Thermal Gradient for Easy Protocol Optimization
The programmable temperature gradient allows you to identify the optimal annealing temperature in a single PCR experiment by letting you test eight incubation temperatures at once.

The gradient feature uses dynamic ramping, which means the temperature gradient forms during ramping and all wells come to the designated temperature at the same time. As a result, the incubation period is the same for all samples, which is crucial for transfer of gradient to nongradient protocols.

The O-ring hermetic seal extends the life of the heating and cooling elements. Condensation from constant heating and cooling can degrade thermal electric modules (TEs), causing them to fail.

Bio-Rad’s Thermal Cyclers include a patented* O-ring seal (red in illustration) that forms an airtight barrier around the TEs, preventing exposure to condensation. This substantially lengthens the life of TEs and guarantees outstanding thermal performance. U.S. patent 7,051,536.

Optimize Reactions in a Single Run

  • Optimize Reactions in a Single Run
  • Optimizing incubation temperatures improves speed and specificity of PCR and real-time PCR, especially with SYBR® Green assays
  • Thermal gradient feature allows optimization of denaturation, annealing, and extension temperatures in 1 experiment
  • Multizone temperature control ensures accuracy and reproducibility for dependable results
  • Dynamic ramping keeps incubation times constant
  • Thermal gradients are available on all of Bio-Rad’s thermal cyclers and real-time PCR systems

Gradient optimization of different PCR reactions

Gradient optimization of different PCR reactions. All reactions were evaluated in a single run. Four different primer sets (A, B, C, and D) were designed and tested for amplification. Arrows indicate the annealing temperature that provided the highest specificity while maintaining good yield. Yellow box indicates optimal temperatures. M, markers; Tm, melting temperature.

PTC Temp Thermal Cycler AR App

Interested in taking an augmented reality (AR) tour to see how the PTC Tempo Thermal Cycler can easily fit into your lab? Download the app now to your mobile device or tablet.

Available on the AppStore Google Play Store

Thermal Cycler Selection Chart

Feature PTC Tempo
PTC Tempo		 C1000 Touch™
C1000 Touch™		 S1000™
S1000™		 T100™
T100™
Peltier-effect technology • • • •
Upgrade to real-time PCR – • – –
Interchangeable sample blocks – • • –
Number of wells 96, deepwell, 48/48, or 384 96, 96 deepwell, 48/48, or 384 96, 96 deepwell, 48/48, or 384 96
Gradient capability • • • •
Graphical user interface • • – •
Fast PCR protocol templates • • • •
Choice of temperature control mode • • • •
Power failure restore • • • •
Programmable ramp rates • • • •
Networking capability • • • –
BR.io Cloud • – – –
Automated lid 96, deepwell, 384 - - -
Remote command set for automation 96, deepwell, 384 - - -
Reports on cycler use and performance • • • •
Heated lid • • • •
Full-skirted plate compatibility • • • –
Wi-Fi • – – –
Security Edition Software to support 21 CFR Part 11 Compliance • – – –

Useful Tools

Related Products

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Specifications

Sample block type
Fixed
Weight 
15 kg (33 lb)
Dimensions (W x D x H) 
28 x 50 x 26 cm (11 x 20 x 10 in)
Power
100–240 V, 50/60 Hz, 850 W max
Electrical approvals
IEC, CE
Memory
4.6 GB
Noise level
50-70 dB at steady state
Lid type
96, Deepwell, 384: Automated; 48/48: Nonautomated
Touchscreen display
8 in. LCD
Programming options
Step-based graphical and automatic
Security features for regulated environments
Password protection and secure mode
Reporting
Exportable run logs and system logs
Temperature control modes
Calculated and block
Onboard operating system
Linux
Communications
Wi-Fi, BR.io, USB A 2.0, Ethernet
Heating and cooling method
Peltier
Lid heating
30–110°C
Temperature range
4–100°C
Thermal accuracy
+/- 0.2°C of programmed target at 90°C
Thermal uniformity
+/- 0.4°C well-to-well within 10 sec of arrival at 90°C
Gradient operational range
30–100°C
Gradient programmable span
1–24°C; 96, Deepwell, 48/48: 8 rows; 384: 16 rows

Ordering

items

Use the filters below to refine results!

22-0142 GXB PTC Tempo 96 Thermal Cycler
(Discontinued) PTC Tempo 96 Thermal Cycler
12015382

96-well, automated lid thermal cycler for PCR with cables; for research use only.

12015392_PTC_Tempo_Deepwell_Thermal_Cycler_Left
(Discontinued) PTC Tempo Deepwell Thermal Cycler
12015392

96-deepwell, thermal cycler for PCR with cables; for research use only.

PTC Tempo_384 Thermal Cycler
(Discontinued) PTC Tempo 384 Thermal Cycler
12015394

384-well, thermal cycler for PCR with cables; for research use only.

PTC Tempo 4848 Thermal Cycler
(Discontinued) PTC Tempo 48/48 Thermal Cycler
12015309

48/48-well, thermal cycler for PCR with cables; for research use only.

Accessories

12019776-prod-sku
(Discontinued) PTC Tempo Thermal Cycler API, 1 License
12019776

For use with the PTC Tempo 96, PTC Tempo Deepwell, or PTC Tempo 384 Thermal Cycler. Each instrument requires 1 license to utilize the instrument API for automation or robotics integration.

12013205
(Discontinued) Ethernet Cable
12013205

A cat-5 ethernet cable for connecting the CFX Opus Real-Time PCR Systems or PTC Tempo Thermal Cyclers to ethernet ports.

(Discontinued) Dual 48/48 Reaction Module Tube Frame for 0.2 ml Tubes
1849000

Tube frame to provide extra support for individual 0.2 ml tubes in the dual 48/48-well reaction modules

12021890
(Discontinued) Tube Frame for 96-Well Reaction Module
12021890

Tube frame to provide extra support for individual 0.2 ml tubes in the 96-well reaction modules

PTC Tempo Thermal Cycler WiFi Adapter
(Discontinued) PTC Tempo Thermal Cycler Wi-Fi Adapter, US Only
12018650

Wi-Fi antenna enables Wi-Fi connection for PTC Tempo Thermal Cycler in the United States.

GXB PTC Tempo Tube Frame
(Discontinued) PTC Tempo Deepwell Tube Frame for 0.5 ml Tubes
12020274

Tube frame to provide extra support for individual 0.5 ml tubes in the PTC Tempo Deepwell Thermal Cycler

Plate Lifter
(Discontinued) Plate Lifter for 384-Well Reaction Module
12014294

Plate lifter to provide support when using 384-well plates with the CFX Opus 384 or PTC Tempo 384

PTC Tempo 48 48 Thermal Cycler
(Discontinued) PTC Tempo Thermal Cycler, Security Edition Software, 1 License
12020564

For use with the PTC Tempo 96, PTC Tempo Deepwell, PTC Tempo 48/48, or PTC Tempo 384 Thermal Cycler. Each instrument requires 1 license to utilize Security Edition features, ideal for supporting 21 CFR Part 11 requirements

Kits & Reagents

(Discontinued) iScript™ cDNA Synthesis Kit, 25 x 20 µl rxns
1708890
25 x 20 µl reactions, includes 5x reverse-transcription reaction mix, iScript reverse transcriptase, nuclease-free water
(Discontinued) iScript™ cDNA Synthesis Kit, 100 x 20 µl rxns
1708891
100 x 20 µl reactions, includes 5x reverse-transcription reaction mix, iScript reverse transcriptase, nuclease-free water
(Discontinued) iScript™ Select cDNA Synthesis Kit, 25 x 20 µl rxns
1708896
25 x 20 µl reactions, includes 5x reaction mix, iScript reverse transcriptase, oligo(dT), random primer mix, gene-specific primer enhancer solution, nuclease-free water
(Discontinued) iScript™ Select cDNA Synthesis Kit, 100 x 20 µl rxns
1708897
100 x 20 µl reactions, includes 5x reaction mix, iScript reverse transcriptase, oligo(dT), random primer mix, gene-specific primer enhancer solution, nuclease-free water
(Discontinued) iTaq™ DNA Polymerase, 250 U (5 U/µl), 50 µl
1708870
250 U (5 U/µl), hot-start polymerase for PCR and real-time PCR, includes 1.25 ml each 10x PCR buffer and 50 mM MgCl2
(Discontinued) iTaq™ DNA Polymerase, 5,000 U (5 U/µl), 1 ml
1708875
5,000 U (5 U/µl), hot-start polymerase for PCR and real-time PCR, includes 25 ml each 10x PCR buffer and 50 mM MgCl2
(Discontinued) iProof GC Master Mix, 100 x 50 µl rxns, 2.5 ml
1725320
100 x 50 µl reactions, premixed PCR reagents, includes 2x master mix (0.04 U/µl), DMSO; suitable for GC-rich templates
(Discontinued) iProof™ High-Fidelity PCR Kit, 50 U (2 U/µl), 25 µl
1725330
50 U, 2 U/µl, iProof polymerase PCR kit includes 5x reaction buffers, MgCl2 solution, DMSO, dNTPs, λ DNA, 1.3 and 10 kb primers, DNA standard
(Discontinued) iProof™ High-Fidelity PCR Kit, 200 U (2 U/µl), 100 µl
1725331
200 U, 2 U/µl, iProof DNA polymerase PCR kit includes 5x reaction buffers, MgCl2 solution, DMSO, dNTPs, λ DNA, 1.3 and 10 kb primers, DNA standard
(Discontinued) iProof™ High-Fidelity DNA Polymerase, 100 U (2 U/µl), 50 µl
1725301
100 U, 2 U/µl, stand-alone enzyme, includes 5x reaction buffers, MgCl2 solution, DMSO
(Discontinued) iProof™ High-Fidelity DNA Polymerase, 500 U (2 U/µl), 250 µl
1725302
500 U, 2 U/µl, stand-alone enzyme, includes 5x reaction buffers, MgCl2 solution, DMSO
(Discontinued) iProof HF Master Mix, 100 x 50 µl rxns, 2.5 ml
1725310
100 x 50 µl reactions, premixed PCR reagents, includes 2x master mix (0.04 U/µl), DMSO; suitable for most templates

Electrophoresis Gel Systems

1704482
(Discontinued) Sub-Cel GT Horizontal Electrophoresis System, 15 x 15 cm tray, with gel caster and casting gates
1704482

Horizontal electrophoresis system, includes 15- and 20-well combs, 15 x 15 cm (W x L) UV-transparent tray, casting gates, gel caster

1704484
(Discontinued) Sub-Cell GT Horizontal Electrophoresis System, 15 x 25 cm tray, with gel caster
1704484

Horizontal electrophoresis system, includes 15- and 20-well combs, 15 x 25 cm (W x L) UV-transparent tray, gel caster

1704468
(Discontinued) Wide Mini-Sub Cell GT Horizontal Electrophoresis System, 15 x 10 cm tray
1704468

Horizontal electrophoresis system, includes 15- and 20-well combs, 15 x 10 cm (W x L) UV-transparent tray

1704469
(Discontinued) Wide Mini-Sub Cell GT Horizontal Electrophoresis System, 15 x 10 cm tray, with gel caster
1704469

Horizontal electrophoresis system, includes 15- and 20-well combs, 15 x 10 cm (W x L) UV-transparent tray, mini-gel caster

1704466
(Discontinued) Mini-Sub Cell GT Horizontal Electrophoresis System, 7 x 10 cm tray
1704466

Mini horizontal electrophoresis system, includes 8- and 15-well combs, 7 x 10 cm UV-transparent tray

1704467
(Discontinued) Mini-Sub Cell GT Horizontal Electrophoresis System, 7 x 10 cm tray, with mini-gel caster
1704467

Mini horizontal electrophoresis system, includes 8- and 15-well combs, 7 x 10 cm (W x L) UV-transparent tray, mini-gel caster

Plastics

(Discontinued) Hard-Shell® 96-Well PCR Plates, high profile, semi skirted, clear/clear
HSS9601
Pkg of 25, clear shell/clear well PCR plate to fit most instruments, rigid 2-component design
(Discontinued) Multiplate™ 96-Well PCR Plates, low profile, unskirted, clear
MLL9601
Pkg of 25, clear PCR plate, one-component polypropylene, can be cut for fewer samples
(Discontinued) Hard-Shell® 96-Well PCR Plates, low profile, thin wall, skirted, white/clear
HSP9601

Pkg of 50, white shell/clear well PCR plate, rigid 2-component design

(Discontinued) Hard-Shell® 96-Well PCR Plates, low profile, thin wall, skirted, green/clear
HSP9641

Pkg of 50, green shell/clear well PCR plate, rigid 2-component design

(Discontinued) Hard-Shell® 96-Well PCR Plates, low profile, thin wall, skirted, blue/clear
HSP9631

Pkg of 50, blue shell/clear well PCR plate, rigid 2-component design

(Discontinued) Hard-Shell® 96-Well PCR Plates, low profile, thin wall, skirted, yellow/clear
HSP9621

Pkg of 50, yellow shell/clear well PCR plate, rigid 2-component design

(Discontinued) Hard-Shell® 96-Well PCR Plates, low profile, thin wall, skirted, red/clear
HSP9611

Pkg of 50, red shell/clear well PCR plate, rigid 2-component design

(Discontinued) Hard-Shell® 96-Well PCR Plates, low profile, thin wall, skirted, white/clear, barcoded
HSP9901

Pkg of 50, white shell/clear well PCR plate, barcoded, 7-digit sequential, Code 128, rigid 2-component design

(Discontinued) Hard-Shell® 384-Well PCR Plates, thin wall, skirted, clear/clear
HSP3801
Pkg of 50, clear shell/clear well PCR plate, rigid 2-component design
(Discontinued) Multiplate™ 48-Well PCR Plates, low profile, unskirted, clear
MLL4801
Pkg of 50, natural PCR plate, 8 x 6 format, one-component polypropylene, can be cut for fewer samples
TCS0803 TN
(Discontinued) 0.2 ml Flat PCR Tube 8-Cap Strips, optical, ultraclear
TCS0803

Pkg of 120, 8-cap strips for 0.2 ml tubes and plates for PCR and optical reactions, ultraclear

(Discontinued) 0.2 ml 8-Tube PCR Strips without Caps, low profile, clear
TLS0801

Pkg of 120, thin-wall polypropylene 8-tube strips for PCR and low-volume reactions, clear (960 tubes)

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