SYBR^® Green

SYBR^® Green Supermixes for Real-Time PCR

Bio-Rad's SYBR^® Green supermixes for real-time PCR are proven formulations for a wide variety of qPCR assays offering reliable, consistent real-time PCR results. Available with our proprietary passive reference dyes, our SYBR^® Green PCR supermixes feature advanced enzymes and buffer formulations to ensure superior speed, sensitivity, and specificity in a wide variety of applications and are compatible with all qPCR protocols and real-time PCR detection systems.

Choose from SYBR^® Green supermixes with antibody-mediated hot-start iTaq™ polymerase or the patented SsoAdvanced™ Sso7d fusion polymerase, featuring an engineered double-stranded (ds) DNA–binding protein for enhanced amplification speed, processivity, and PCR inhibitor tolerance.

Proven Chemistry for Dye-Based Real-Time PCR Assays

SYBR^® Green dye is an economical option for dye-based real-time PCR assays. Although dye-based assays are not suitable for multiplex PCR, for singleplex assays, SYBR^® Green yields reproducible qPCR results without the need for expensive and labor-intensive fluorescent probe design. SYBR^® Green real-time PCR assays are ideally suited for high-throughput screening programs designed to detect single targets in many samples, with applications in applications in clinical research, gene expression studies, population genetics, and environmental and food safety testing.

One potential problem with SYBR^® Green chemistry is the possibility of nonspecific product detection. Fortunately, a melt curve analysis provides a simple way of determining the presence or absence of spurious PCR products: a post-amplification sample containing only the target product will yield a single sharp peak in a plot of the second derivative of fluorescence vs. temperature, whereas the presence of side-products will yield multiple peaks. Bio-Rad real-time PCR detection systems feature simple programming to automatically perform a melt curve analysis conveniently after the main amplification/quantification run.

SYBR^® Green Supermix Applications

SYBR^® Green PCR supermixes are available for a wide range of applications and optimized for most real-time PCR amplification protocols. SYBR^® Green applications include:

• Gene expression studies
• Genomics applications
• Genotyping
• Forensics
• Mutation detection
• Food safety testing
• GMO analysis
• Pathogen detection

SYBR^® Green can also be used to visualize DNA in electrophoresis gels. SYBR^® Green has a high specificity for dsDNA and is especially useful when RNA or ssDNA may also be present in the sample. SYBR^® Green displays very low background fluorescence, and its excitation and emission spectra are well matched to the blue light or UV sources and filter sets in commonly used imaging instruments. SYBR^® Green is also a good substitute for the common DNA stain ethidium bromide, a potential human mutagen; SYBR^® Green is safer to work with and poses no waste disposal problems.