1x96-well filter plate, x-Plex assay format that includes premixed coupled beads, detection antibodies, and lysates for detecting phosphorylated Akt (Ser473), CREB (Ser133), GSK-3α/β(Ser21/Ser9), IGF-IR (Tyr1131), IkappaB-α(Ser32/Ser36), IRS-1 (Ser636/Ser639), p70 S6 kinase (Thr421/Ser424), p90RSK (Thr359/Ser363), STAT3 (Ser727), requires phosphoprotein reagent and cell lysis kit offered separately