1x96-well filter plate, x-Plex assay format that includes premixed coupled beads, detection antibodies, and lysates for detecting phosphorylated GSK-3α/β(Ser21/Ser9), HSP27 (Ser78), IkappaB-α(Ser32/Ser36), JNK (Thr183/Tyr185), p38 MAPK (Thr180/Tyr182), requires phosphoprotein reagent and cell lysis kit offered separately