1 X 96-well filter plate, x-Plex assay format that includes premixed coupled magnetic beads, detection antibodies, standards, reagents, and diluents for detecting human cytokines IL-1α, IL-2Rα, IL-12 (p40), IL-16, IL-18, IFN-α2, MCP-3, MIF, MIG, TNF-ß, all in one kit.