1 X 96-well flat bottom plate, x-Plex assay format that includes premixed coupled magnetic beads, detection antibodies, standards, reagents, and diluents for detecting human cytokines IL-1ß, IL-1ra, IL-4, IL-5, IL-6, IL-12 (p70), IL-13, Basic FGF, IFN-ƴ, TNF-α, all in one kit.