QXDx Automated Droplet Generator #12008019

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Overview

Automated droplet generator for probe-based digital PCR application, for use with the QXDx AutoDG ddPCR System

Description

Description

Use the QXDx Automated Droplet Generator in the digital PCR in vitro diagnostics (IVD)* workflow to partition 96 digital PCR reactions into 20,000 nanoliter-sized droplets prior to the PCR reaction in under 45 minutes. The Automated Droplet Generator is part of the QXDx AutoDG ddPCR System, Bio-Rad's Droplet Digital System cleared for use in a regulatory environment, which provides precise and absolute quantitation of target DNA or RNA molecules.

Features and Benefits

  • FDA-Cleared — the first FDA-cleared ddPCR system giving the industry a quality product with great sensitivity and reproducibility
  • Absolute quantification — absolute count of target DNA copies per input without the need for standard curves
  • Highest precision and sensitivity — enriched template concentration in target-positive droplets enabling higher precision and sensitivity than NGS and quantitative PCR (qPCR)
  • High throughput — simple and easy-to-use workflow with 96-reaction throughput
  • Removal of PCR efficiency bias — error rates are reduced by removing the reliance on amplification efficiency of qPCR due to high tolerance of PCR inhibitors

Related Products

The QXDx AutoDG ddPCR System is used and validated with the following thermal cycler specifications:

  • Accuracy: ±0.2°C
  • Uniformity: ±0.4°C well-to-well within 10 sec
  • Adjustable ramp capability with required ramp rate: up to 2°C/sec
  • Temperature range: 0–100°C

The C1000 Touch Thermal Cycler with 96–Deep Well Reaction Module (1851197) was used during the development and validation efforts of all QXDx products.

Related Categories

Supporting Documents

QXDx Droplet Reader and QuantaSoft Software Instruction Manual

Online Resources

Introduction to Digital PCR

*Legal Notice
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. Purchase of the product includes a limited, non-transferable right under such intellectual property for use of the product for internal research and diagnostic purposes only. No rights are granted for use of the product for commercial applications of any kind, including but not limited to manufacturing, quality control, or commercial services, such as contract services or fee for services. Information concerning a license for such uses can be obtained from Bio-Rad Laboratories. It is the responsibility of the purchaser/end user to acquire any additional intellectual property rights that may be required.