Zika, Dengue, and Chikungunya (ZDC) Real-Time PCR Assays

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Overview

Bio-Rad Laboratories developed the ZDC Multiplex RT-PCR Assay (Research Use Only) for the simultaneous detection of Zika, dengue, and chikungunya viral RNA.

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Description

Description

Bio-Rad Laboratories developed the ZDC Multiplex RT-PCR Assay (Research Use Only) for the simultaneous detection of Zika (ZIKV), dengue (DENV), and chikungunya (CHIKV) viral RNA. This assay has been wet-lab validated using Bio-Rad’s CFX96™ and Thermo Fisher Scientific’s ABI 7500 Real-Time PCR Detection Systems. The assay is also compatible with most qPCR systems with at least 4-channel multiplex capability.

Convenient one-step assay with all controls

The ZDC Multiplex RT-PCR Assay is a one-step RT-PCR assay for the multiplex detection of Zika, dengue, and chikungunya viral RNA by real-time PCR. The assay includes all the controls needed for reliable results, thus providing researchers with a robust tool to identify ZIKV, DENV, and CHIKV nucleic acids in RNA extracted from a wide range of sample types.

Product Features

  • Enough to perform 200 tests
  • One-step procedure — RT + amplification are performed in a single tube
  • 3-plex assay for the simultaneous detection of ZIKV, DENV (DENV1, DENV2, DENV3, and DENV4), and CHIKV RNA
  • Internal control included for use as a fourth target in the multiplex assay
  • Wet-lab validated on the CFX96 Touch and ABI 7500 Real-Time qPCR Systems
  • Compatible with the most popular qPCR systems on the market
  • Compatible with RNA extracted from multiple starting materials (serum, plasma, serum-free body fluids, cerebrospinal fluids, and mosquitoes)
  • High-standard GMP manufacturing procedures for the key components of the kit
  • RNase P assays for internal sample controls; specifically designed for 5-plex experiments on CFX96 and ABI 7500 Real-Time qPCR Systems

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12003818
Pkg of 1, includes ZDC Multiplex RT-PCR Assay, positive and internal control RNAs, iTaq™ Universal Probes One-Step Kit, iScript™ Reverse Transcriptase, ZDC buffer, and nuclease-free water; sufficient to perform 200 tests

Kits & Reagents

Kits & Reagents

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(Discontinued) ZDC Buffer T
12005062
Pkg of 5, 1.8 ml tubes, ZDC Buffer T for use with ZDC Multiplex RT-PCR Assay
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12004860
Pkg of 1, 5 ml tube, ZDC Clear Solution, for use with RNase P Assay and ZDC Multiplex RT-PCR Assay

RNaseP Assay

RNaseP Assay

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12004601
Pkg of 1, vial, 440 µl of 12.5x concentrated primers/probe solution
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12004602
Pkg of 1, vial, 440 µl of 12.5x concentrated primers/probe solution

Documents

Documents

Number Description Options
12003818
ZDC (Zika, Dengue, and Chikungunya) Multiplex RT-PCR Assay
6978
ZDC Multiplex RT-PCR Assay Flier