Herpes simplex virus (HSV)

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Herpes simplex (HSV)

Overview

Herpes Simplex Virus (HSV)

Herpes Simplex Virus 1/2 DFA

Pathfinder® HSV Identification in Direct Clinical Specimen Results by Investigator

  TP FP FN TN Inadequate Total No. (%) Total
Pathfinder Reagents + +    
Cell Culture Method + +    
Investigator A 34 0 6 18 11 (16%) 69
Investigator B 21 1 6 18 3 (6%) 49
In-house 26 0 8 7 6 (13%) 47
Total 81 1 20 43 20 165
Sensitivity = TP/(TP + FN) = 80% Specificity = TN/(TN + FP) = 98%


HSV Identification in Direct Clinical Specimen Results by Sex and Site of Lesion

      Predictive Value
Site of Lesion Male Female Sensitivity
TP/(TP + FN)
Specificity
TN/(TN + FP)
Positive
TP/(TP + FP)
Negative
TN/(TN + FN)
Genital 69   79%
38/48
95%
20/21
97%
38/39
67%
20/30
Genital   43 81%
25/31
100%
12/12
100%
25/25
67%
12/18
Anal 8 3 71%
5/7
100%
4/4
100%
5/5
67%
4/6
Other 10 11 86%
12/14
100%
7/7
100%
12/12
78%
7/9
Unknown 1          
Total 88 57  


Note: The calculations in the two tables are based on comparison to cell culture as the reference method. TP = true positive, FP = false positive, FN = false negative, TN = true negative
.

Herpes Simplex Virus (HSV)

Herpes Simplex Virus 1 and 2 IgG EIA Kit

Interpretation of Bio-Rad HSV 1 and 2 IgG Results

Index Interpretation
< 0.9 Negative for anti-HSV; presumed no previous exposure
≥ 0.9 <1.1 Equivocal
≥ 1.1 Positive for anti-HSV; presumed exposure to HSV type 1 or type 2


Bio-Rad HSV 1 and 2 IgG

Comparative Assay Positive Equivocal Negative   95% Cl
Type 1 HSV
Positive 158 (36) 0 0 Relative Sensitivity 97.1–100 **
Negative 11 (2) 8 30 (2) Relative Specificity* 59.6–86.7 ***
        Overall Agreement* 91.3–97.6 ***
Type 2 HSV
Positive 153 (35) 0 0 Relative Sensitivity 97.6–100 **
Negative 16 (3) 8 30 (2) Relative Specificity* 51.5–79.0 ***
        Overall Agreement* 88.2–95.7 ***


   * Excluding equivocal results.
 ** Calculated by the Exact Method.
*** Calculated by the Normal Method
 ( ) Female donors of childbearing age.

Results of tests of 207 specimens (100% frozen). Forty (19%) of the specimens tested were obtained from women of childbearing age.

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Bio-Rad’s Pathfinder HSV 1 and 2 DFA kit provides direct antigen detection and differentiation of herpes simplex virus (HSV) types 1 and 2 using fluorescein-conjugated monoclonal antibodies. Two results are available from one test in one hour. This test kit allows room temperature incubation and confirmation of cell culture isolates. The monoclonal antibody-based test can be used to directly stain clinical specimens or tissue culture cells. Flexible ordering options are available.

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Pathfinder HSV Types 1 and 2

25215
Direct antigen detection and differentiation of Herpes simplex virus (HSV) types 1 and 2 in direct clinical specimens and cell culture isolates (50 tests)

25215 /templatedata/internet/product_SKU/data/CDG/Microbiology/Direct-Antigen-Detection/25215_0921120859473.xml 25215 09/21/09 12:08 PM en_US true internet/product_SKU 2019-05-04 12:32:18 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN templatedata/internet/product_SKU/data/CDG/Microbiology/Direct-Antigen-Detection/25215_0921120859473.xml /default/main/bio-rad/WORKAREA/default jrtv5eoa 2017-11-14 07:06:41 true jrtv5eoa templatedata/internet/product_SKU/data/CDG/Microbiology/Direct-Antigen-Detection/25215_0921120859473.xml CDG N /default/main/bio-rad en 12/31/37 11:59 PM 1555633008356 2019-05-04 12:32:18 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/CDG/Microbiology/Direct-Antigen-Detection/25215_0921120859473.xml 25215-pathfinder-hsv-types-1-2 1 25215 Pathfinder HSV Types 1 and 2 Kit Pathfinder HSV Types 1 and 2 Direct antigen detection and differentiation of Herpes simplex virus (HSV) types 1 and 2 in direct clinical specimens and cell culture isolates (50 tests) 50 monoclonal antibody-based direct immunofluorescence assays to detect and differentiate herpes simplex virus types 1 and 2 Direct antigen detection and differentiation of Herpes simplex virus (HSV) types 1 and 2 in direct clinical specimens and cell culture isolates (50 tests) Pathfinder HSV Types 1 and 2 ,VN RepEx 25215 50 monoclonal antibody-based direct immunofluorescence assays to detect and differentiate herpes simplex virus types 1 and 2 09/21/09 12:08 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en CDG /CDG/Microbiology/Direct-Antigen-Detection N 0 false null false $ null false false true true false USD true false true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=25215&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=25215&CartQuote=QT&quantity= 2469 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=25215&AddToDefaultWishList=true&quantity= 41116100 null 25215
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Pathfinder HSV Types 1 and 2 Specimen Slides

30494
Collection slides for use with Herpes simplex virus (HSV) types 1 and 2 test kits (90 slides)

30494 /templatedata/internet/product_SKU/data/CDG/Microbiology/Direct-Antigen-Detection/30494_0921120859491.xml 30494 09/21/09 12:08 PM en_US true internet/product_SKU 2019-05-04 12:32:08 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN templatedata/internet/product_SKU/data/CDG/Microbiology/Direct-Antigen-Detection/30494_0921120859491.xml /default/main/bio-rad/WORKAREA/default jrtv5enq 2017-11-14 07:06:41 true jrtv5enq templatedata/internet/product_SKU/data/CDG/Microbiology/Direct-Antigen-Detection/30494_0921120859491.xml CDG N /default/main/bio-rad en 12/31/37 11:59 PM 1555633008471 2019-05-04 12:32:08 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/CDG/Microbiology/Direct-Antigen-Detection/30494_0921120859491.xml 30494-hsv-collection-slide 1 30494 Pathfinder HSV Types 1 and 2 Specimen Slides Pathfinder HSV Types 1 and 2 Specimen Slides Collection slides for use with Herpes simplex virus (HSV) types 1 and 2 test kits (90 slides) Box of 90 slides to mount specimens for Pathfinder HSV Types 1 and 2 Kit Collection slides for use with Herpes simplex virus (HSV) types 1 and 2 test kits (90 slides) ,VN RepEx 30494 Box of 90 slides to mount specimens for Pathfinder&trade; HSV Types 1 and 2 Kit 09/21/09 12:08 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en CDG /CDG/Microbiology/Direct-Antigen-Detection N 0 false null false $ null false false true true false USD true false true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=30494&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=30494&CartQuote=QT&quantity= 2469 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=30494&AddToDefaultWishList=true&quantity= 41116100 null 30494
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Pathfinder Mounting Medium

30693
2.75 mL of mounting medium for slides

30693 /templatedata/internet/product_SKU/data/CDG/Microbiology/Direct-Antigen-Detection/30693_0921120858972.xml 30693 09/21/09 12:08 PM en_US true internet/product_SKU 2019-05-04 12:32:02 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN templatedata/internet/product_SKU/data/CDG/Microbiology/Direct-Antigen-Detection/30693_0921120858972.xml /default/main/bio-rad/WORKAREA/default jrtv5ens 2017-11-14 07:06:41 true jrtv5ens templatedata/internet/product_SKU/data/CDG/Microbiology/Direct-Antigen-Detection/30693_0921120858972.xml CDG N /default/main/bio-rad en 12/31/37 11:59 PM 1555633008588 2019-05-04 12:32:02 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/CDG/Microbiology/Direct-Antigen-Detection/30693_0921120858972.xml 30693-pathfinder-mounting-medium 1 30693 Pathfinder Mounting Medium Pathfinder Mounting Medium 2.75 mL of mounting medium for slides 2.75 mL of mounting medium for slides 2.75 mL of mounting medium for slides /webroot/web/images/cdg/products/microbiology/sku_thumb/global/cmd_30693_tn.jpg /webroot/web/images/cdg/products/microbiology/sku_view/global/cmd_30693_view.jpg /webroot/web/images/cdg/products/microbiology/sku_feature/global/cmd_30693_feature.jpg Pathfinder Mounting Medium ,VN RepEx 30693 2.75 mL of mounting medium for slides 09/21/09 12:08 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en CDG /CDG/Microbiology/Direct-Antigen-Detection N 0 false null false $ null false false true true false USD true false true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=30693&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=30693&CartQuote=QT&quantity= 2417 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=30693&AddToDefaultWishList=true&quantity= 41116100 null 30693
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J-109A Herpes Simplex Virus 1/2 Product Sheet Click to download [ Add to Cart (Free) ]

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