Anti-β2 Glycoprotein I (anti-β2GPI) Assays

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Anti-beta2 Glycoprotein I (anti-beta2GPI) Assays

Overview

Anti-β2-Glycoprotein 1 Assays

Anti-β2-Glycoprotein 1 Autoimmune EIA Overview

Anti-β2-Glycoprotein 1 (anti-β2GP1) Testing

The Anti-β2GP1 assay serves to assist in the diagnosis of antiphospholipid syndrome. This diagnosis has important implications for the treatment and prediction of recurrent thrombosis and recurrent miscarriage.

The Bio-Rad Anti-β2-Glycoprotein 1 Test Kits are enzyme-linked immunosorbent assays (ELISA) for the detection and semi-quantitative determination of anti-β2GP1 IgG, IgM, or IgA antibodies in human serum or plasma. The results are reported in G, M and A units. The Bio-Rad Anti-β2-Glycoprotein 1 Test Kits are intended to be used by clinical (hospital and reference) laboratories.

Product Highlights

  • Specific determinations of IgG, IgM, and IgA anti-β2GP1 antibodies
  • Flexible multi-point or single-point calibration options
  • Better regression analysis with distinct calibrators
  • Built-in quality control with undiluted controls and calibrators

 

Anti-Cardiolipin Assays

Anti-Cardiolipin Autoimmune EIA

Pre-Test Setup Instructions


Step 1. Bring all reagents to room temperature (18–26°C).


Step 2. Prepare the Wash Solution.


Step 3. Make 1:50 working solutions by diluting 10 µL of patient's serum positive/negative controls, and 3 calibrators in 490 µL of diluent.


Test Procedure


Step 1. Apply 100 µL diluted patient samples, controls, and calibrator to wells.

Step 2. Incubate wells for 30–40 minutes at room temperature (18–26°C).


Step 3. Wash wells 4x with ~250 µL of wash solution.Remove all liquid before proceeding.


Step 4. Apply 100 µL HRP conjugate to all wells. Discard excess transferred HRP after use.


Step 5. Incubate wells for 30–40 minutes at room temperature (18–26°C).


Step 6. Wash wells 4x with ~250 µL of wash solution. Remove all liquid before proceeding.


Step 7. Develop color by adding 100 µL substrate to each well. Discard excess transferred substrate after use.


Step 8. Incubate for 30–40 minutes at room temperature (18–26°C)


Step 9. Stop color development by adding 100 µL of stop solution to each well.


Step 10. Read results within 30 minutes with an EIA reader set to 450 nm.

Anti-Cardiolipin Assays

Anti-Cardiolipin Autoimmune EIA

aPL Testing Algorithm: Antiphospholipid Antibody Screening Procedure

Screen with aCL and/or aPS (IgG, IgM, IgA)*

Test for anti-β2 GPI (IgG, IgM, IgA)*

Repeat testing in at least six weeks for seroconversion or antibody persistance


* Screen for each isotype. Polyvalent screen not recommended.

APS = Antiphospholipid syndrome
aCL = Anti-cardiolipin
aPS = Anti-phosphatidylserine
β2 GPI = β-2 glycoprotein 1 (cofactor)


Cut-Off Value

  • aCL IgG: <23 GPL units
  • aCL IgA: <22 APL units
  • aCL IgM: <11 MPL units
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Anti-Beta 2 Glycoprotein I (anti-β2GPI) assays are intended for the semi-quantitative determination of anti-β2GPI antibodies in human serum or citrated plasma (3.2 % sodium citrate).

Bio-Rad’s Anti-β2 Glycoprotein I (anti-β2GPI) Assays feature common protocols and common reagents. All reagents and reagent bottle caps are color coded for ease of use. Test operation is further streamlined with breakaway wells, easy automation, a linear calibration curve and flexible single-point and multi-point calibration options.

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Anti-β2 Glycoprotein I IgG EIA Kit

425-2060
Semi-quantitative enzyme immunoassay for detection of anti-beta2 glycoprotein I IgG antibodies (96 tests)

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Anti-β2 Glycoprotein I IgM EIA Kit

425-2080
Semi-quantitative enzyme immunoassay for detection of anti-beta2 glycoprotein I IgM antibodies (96 tests)

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Anti-β2 Glycoprotein I IgA EIA Kit

425-2100
Semi-quantitative enzyme immunoassay for detection of anti-beta2 glycoprotein I IgA antibodies (96 tests)

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