48-Well PCR Plates

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48-Well Plates for PCR

Overview

PCR Consumables Selection Wizard

Instrument Compatibility
Unskirted PCR Plate Comparison Multiplate 48- and 96-Well Unskirted PCR Plates, Full-Height Multiplate 48- and 96-Well Unskirted PCR Plates, Low-Profile  
Catalog number MLP-xxxx MLL-xxxx  
Well height 20.70 mm 15.50 mm  
PCR reaction volume 5–125 µl 5–125 µl
Maximum well volume 300 µl 200 µl  
Thermal Cycler Compatibility  
Bio-Rad C1000/S1000
MJ Mini
Bio-Rad DNA Engine, DNA Engine Tetrad and Tetrad 2, DNA Engine Dyad, and Dyad Disciple
Bio-Rad iCycler and MyCycler  
Bio-Rad PTC-100
ABI GeneAmp cyclers with regular blocks  
ABI GeneAmp cyclers with fast blocks  
Eppendorf Mastercycler series
Real-Time PCR Detection System Compatibility  
Bio-Rad CFX96  
MiniOpticon
Bio-Rad iQ5, iCycler iQ, MyiQ    
Bio-Rad Chromo4
Bio-Rad DNA Engine Opticon and Opticon 2  
ABI systems with regular blocks (7300, 7500, 7900HT, etc.)  
ABI fast systems (7500 Fast, 7900HT Fast, StepOne, StepOnePlus, etc.)  
Eppendorf Mastercycler ep realplex
Stratagene Mx series  
Other Instrument Compatibility  
ABI DNA Sequencers (3700, 3730, etc.)  
Idaho Technology LightScanner
Optical Guide

Well Color Effect on Signal Strength

Well color effect on signal strength

White well:

  • Increases fluorescent signal for maximizing detection sensitivity
  • Prevents fluorescent interference from adjacent wells or a contaminated cycler

Clear well:

  • Transparent wells for easy sample handling
  • Low signal variability for consistent results

Black well:

  • Lower signal strength for FP (fluorescence polarization) and other assays where reflected light must be minimized

Sealer Effect on Signal Strength

Sealer effect on signal strength

Optical flat cap strips:

  • Vapor-tight sealing for tube strips or 96-well plates
  • Suitable for PCR volumes >5 µl

Microseal® 'B' adhesive seals, optically clear:

  • A convenient sealing film for 96- or 384-well plates
  • Higher optical transmission than optical caps
  • Suitable for PCR volumes >10 µl

Clear Chill-out™ liquid wax:

  • No heated lid or lid pressure is required for PCR
  • Higher optical transmission than optical caps
  • Solidified below 10°C to prevent spilling and aerosol formation
High-Profile vs. Low-Profile

 

There are two common well heights for PCR tubes and plates:

  1. High-profile (full-height; around 20.7 mm) wells are “standard” and fit in most classical thermal cyclers and real-time PCR detection systems, such as Bio-Rad's iCycler®, iQ™ series real-time PCR detection systems, and Applied Biosystems' regular PCR systems and DNA sequencers.
  2. Low-profile (around 15.5 mm) wells are the newer design that reduces the potential for condensate formation and offers advantages for light capture in fluorescence assays, low-volume reactions, and fast PCR. They have been recommended for newer instruments such as Bio-Rad's C1000 Touch™ and S1000™ thermal cyclers, CFX series real-time PCR detection systems, and Applied Biosystems' fast PCR systems.

Instruments with adjustable lids can usually use both high-profile and low-profile wells. These instruments include Bio-Rad's 1000-series and DNA Engine® series thermal cyclers.

PCR Seals Ideal for NGS Library Preparation

Our foils and optically clear seals have a strong adhesive that minimizes sample evaporation and peels off easily for simple sample retrieval.

Optically Clear Adhesive Seals


Microseal 'B' PCR Plate Seals

Optical seal – same seal can also be used for qPCR, including qPCR-based library quantification assays
Widely adopted for research – Bio-Rad’s most popular seal

Foil Adhesive Seals


Microseal 'F' PCR Plate Seals

Ideal for sample storage – highly solvent resistant and suitable for storage down to –80°C
Pierceable aluminum – easily retrieve sample by piercing with pipette tip

Unskirted 48-well PCR plates made of polypropylene for low protein binding are easily cut with scissors if less than a whole plate is needed. Available in low-profile and high-profile versions.


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Description

Our unskirted Multiplate™ 48-Well PCR Plates are constructed of polypropylene for low protein binding and are easily cut with scissors if less than a whole plate is needed. These PCR microplates are compatible with older Bio-Rad thermal cyclers and systems, the T100™, C1000 Touch™, and S1000™ Thermal Cyclers, and CFX Real-Time PCR Detection Systems. Use the PCR Plastics Selector to determine individual plate compatibility with other instruments.

Features and Benefits of 48-Well PCR Plates

  • Unskirted plates are available in both high-profile and low-profile heights, permitting compatibility with all styles of thermal cyclers and real-time PCR systems
    • Low-profile plates are compatible with all CFX Systems
    • High-profile plates are only compatible with deep-well blocks in the CFX96 Touch™ Real-Time PCR Detection System
  • Compatible with a wide variety of sealing systems
  • Plates are free of DNase, RNase, and human DNA

Related Products

The semi-automated PX1 PCR Plate Sealer delivers reliable uniform sealing of a range of seal types on different styles of microplates. This programmable sealer minimizes sample evaporation and provides consistency.

Related Categories

Online Resources

PCR Plastics Selector


Documents

Number Description Options
6090 Amplification Reagents and Plastics Brochure Click to download [ Add to Cart (Free) ]

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

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