Using Mixed-Mode Selectivity to Address Purification Challenges


April 14, 2014
Xuemei He, PhD
Senior Staff Scientist, Process Chromatography Applications
Bio-Rad Laboratories

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As the structures of novel biotherapeutics become more diverse, new purification schemes are emerging to effectively address purification challenges. Mixed-mode chromatography media, with their unique selectivities, are emerging as powerful tools for the removal of product-related impurities. In this talk we discuss ligand-protein interactions and present several applications of mixed-mode chromatography, with a special focus on orthogonal approaches used to effectively remove closely related species while reducing feed manipulation and buffer consumption. Lastly, we will present proven approaches to accelerate method development by fine-tuning selectivity with minimal experimentation. The talk will highlight several cases spanning a range of proteins.

Topics include:

  • Understanding mixed-mode chromatography basics and how to modulate ligand/protein interactions
  • Developing methods with the least amount of experimentation
  • Addressing difficult purification challenges using new selectivities