Droplet Digital PCR (ddPCR)
Droplet Digital™ PCR (ddPCR™) is a breakthrough technology that provides ultrasensitive nucleic acid detection and absolute quantification. It is highly effective for resolving low abundance targets, such as allelic or structural variants, that are below the level of detection of other platforms.
With advanced multiplexing offerings, ddPCR technology maximizes the number of targets analyzed per well without sacrificing performance.
Digital PCR Assays & Kits
Bio-Rad offers a comprehensive portfolio of Digital PCR Assays and Kits for numerous applications including Mutation Detection, Copy Number Determination, Genome Edit Detection, Gene Expression, Expert Design Assays, Residual DNA Quantification and Library Quantification.
Digital PCR Assays
Get the most out of your experiment with assays designed specifically for Droplet Digital PCR. Simply enter your target of interest or sequence and order a ddPCR Assay.
ddPCR Microsatellite Instability (MSI) Kit
The only kit designed to analyze both plasma and FFPE tissue, it provides highly sensitive and reproducible results across all relevant cancer samples without the need for normal tissue. Automated analysis and a streamlined workflow make this the premier MSI solution.
QX600 Droplet Digital PCR System
Since its introduction, ddPCR technology has advanced critical fields of research through unmatched sensitivity, precision, and absolute quantitation. Now the QX600 System provides advanced, 6-color multiplexing to maximize the number of biomarkers interrogated per well, allowing the preservation of precious samples and reducing lab burden.
Digital PCR Systems & Software
Bio-Rad’s Droplet Digital PCR Systems provide ultrasensitive and absolute nucleic acid quantification.
Digital PCR Consumables & Reagents
Purchase supermixes, droplet generation oil, cartridges, gaskets, plates & seals, plus pipette tips, and more, designed for Droplet Digital PCR Systems.
QXDx Products for In Vitro Diagnostics (IVD)
QXDx AutoDG ddPCR System
- First FDA-cleared ddPCR system for use with QXDx BCR-ABL %IS Kit
- Ultra-low quantification without standard curves
- Droplet partitioning reduces amplification bias
- Automated droplet generator, 96-reaction throughput
QXDx AutoDG Consumable Pack & QXDx Droplet Reader Oil Pack
Reagents and consumables for the automated IVD ddPCR System.
QXDx BCR-ABL %IS Kit
An FDA-cleared/CE-marked digital PCR test kit intended to monitor patients with Chronic Myeloid Leukemia (CML) with reported results in both International Scale and MR values.
ddPCR Certified Kit Provider Partnership Program
Partnering with Bio-Rad for ddPCR Test Kit Development
Bio-Rad partners with test kit providers to develop Droplet Digital™ PCR (ddPCR) assays and diagnostic test kits. We help kit manufacturers to develop and commercialize IVD and registered ddPCR test kits using our Droplet Digital PCR platform. We can help you with assay design, in-licensing, co-development, and co-marketing opportunities.
Droplet Digital PCR Technology
Based on water-emulsion droplet technology, Droplet Digital PCR fractionates a DNA sample in 20,000 droplets. PCR amplification of the template subsequently occurs in each individual droplet, and counting the positive droplets gives precise, absolute target quantification.
Read more about Droplet Digital PCR technology or learn how to transition your assay from qPCR to ddPCR.
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DNA sample with target sequence is partitioned into droplets
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Target and background DNA are randomly distributed among 20,000 droplets
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Target sequence is amplified by end-point PCR in each droplet
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Positive droplets are counted to give precise quantification of target sequences in sample
Benefits of Droplet Digital PCR
- Absolute quantification — provides an absolute count of target DNA copies per input sample without the need for standard curves
- Highest precision and sensitivity — enriched template concentration in target-positive droplets allows higher precision and sensitivity than real-time PCR
- Removal of PCR efficiency bias — error rates are reduced by removing the reliance on amplification efficiency of qPCR
- Explore Absolute Quantification with Droplet Digital PCR, download the White Paper on Molecular Counting.
Droplet Digital PCR Applications
Copy number variation (CNV)
Accurate CNV quantification in single wells.
Next generation sequencing
Amplification and accurate quantification of NGS libraries with validation of sequencing results.
Detection of rare sequences in cancer and disease
Detection of mutations and viral load below levels detectable by current tests.
Gene expression analysis
Detection of rare mRNAs and miRNAs with rapid turnover.
Cell & Gene Therapy
ddPCR assays for a range of applications including CAR transgene quantification.
Pathogen detection and microbiome analysis
Viral load and pathogen detection in organ transplants, soil, and food.
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Hematology-Oncology
Hematology-oncology solutions provide precise and accurate results without the need for standard curves.
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Wastewater-Based Epidemiology
ddPCR assays for unlocking the full potential of wastewater testing and surveillance as a public health tool.
Download the Droplet Digital PCR Applications Guide for in-depth information about setting up experiments.
Bio-Rad is the Trusted Choice for Digital PCR
Over a Decade of Droplet Digital PCR Innovation and Breakthroughs
When it comes to digital PCR, in particular Droplet Digital PCR (ddPCR), Bio-Rad stands as a trusted leader with a track record that speaks for itself. With over 10 years of continuous innovation in digital PCR, and with 8,000+ publications utilizing our ddPCR technology, Bio-Rad has established its reputation as a reliable and respected provider.
8,000+ Publications and Counting
Browse the growing list of cited peer-reviewed journal articles in Droplet Digital PCR research.
Empower Your Research with ddPCR Technology
Our commitment to advancing the field of molecular diagnostics is reflected in the accuracy, sensitivity, and reliability of our ddPCR solutions, making Bio-Rad the trusted choice for researchers worldwide. Join the growing community of scientists who rely on Bio-Rad's ddPCR technology for their groundbreaking discoveries.
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