ddPCR Mutation Detection Assays

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Overview

Bio-Rad’s ddPCR Mutation Detection Assays enable highly sensitive detection of mutations present at very low frequencies in a large excess of wild-type (WT) background DNA.

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Description

Description

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ddPCR Mutation detection assays can be found and purchased on Bio-Rad.com/digital-assays. A wet-lab validated mutation detection assay is returned if available, or an assay is designed instantly for your target. Wet-lab validated mutation detection assays detect mutant DNA present at 0.1% in a background of WT DNA in one well. An even better sensitivity of 0.005–0.1% can generally be achieved by using multiple reaction wells. Browse list of wet-lab validated Mutation Detection Assays.

Mutation detection assays can be designed instantly for over 4 million mutations, listed in the COSMIC database, on Bio-Rad’s Digital Assay Site. Built by the experts in digital PCR at Bio-Rad's Digital Biology Center, the Bio-Rad Digital Assay Site design engine takes into consideration everything from GC content and Tm, to primer placement and probe length, even genome specificity when designing an assay for you.

Key Benefits

  • Wet-lab validated assays available for more than 200 targets
  • Wet-lab validated assays guaranteed to work when used as instructed
  • Validation data available for review and download
  • Assays designed instantly for targets and sequences for which validated assays are not available
  • Suitable for use with circulating tumor DNA, liquid biopsy, and FFPE and other tissue samples
  • Orderable in single-tube or two-tube formats
  • Positive control sequences provided

Sample Data



 

High sensitivity of PrimePCR ddPCR mutation detection assays provide precise quantification of JAK2 V617F mutation in the presence of wild-type DNA. A, 2-D fluorescence amplitude plot shows duplicate wells of a mixed mutant: wild-type sample across multiple percentage mutant blends. The black cluster on the plot represents the negative droplets, the green cluster represents the droplets that are positive for wild-type DNA only, the blue cluster represents the droplets that are positive for mutant DNA only, and the orange cluster represents the droplets that are positive for both wild-type and mutant targets. B, 2-D fluorescence amplitude plot shows duplicate wells of a wild-type–only sample. C, fractional abundance plot shows the percentage frequency (orange markers) of the mutant DNA in a wild-type DNA background. All error bars are generated by QuantaSoft software and represent the 95% confidence interval.

 

Packaging Options

ddPCR Mutation Detection Assays are available in single tube or two-tube formats.

Single-tube format

The single-tube formats of the ddPCR Mutation Detection Assays contain a set of primers, a mutant, and wild-type probe. The single tube format assays are 20X concentrated, ready-to-use-primer-probe mix optimized for use with ddPCR Supermix for Probes (no dUTP). Each assay contains 18 uM each primer and 5uM each probe. The single-tube formats provide added convenience as the mutant and WT probes are pre-mixed and allow for the addition of an extra sample in the ddPCR reaction.

Catalog# Product Name Reaction Size
10049550 ddPCR MUT FAM+HEX Assay, Validated 200
10049551 ddPCR MUT FAM+HEX Assay, Validated 1000
10049552 ddPCR MUT FAM+HEX Assay, Validated 2500
10049047 ddPCR MUT FAM+HEX Assay 200
10049048 ddPCR MUT FAM+HEX Assay 1000
10049049 ddPCR MUT FAM+HEX Assay 2500
12005581 ddPCR MUT Cy5+Cy5.5 Assay 200
12005580 ddPCR MUT Cy5+Cy5.5 Assay 1000
12005579 ddPCR MUT Cy5+Cy5.5 Assay 2500

Two-tube formats

The mutant and WT probes are provided separately.

Catalog# Product Name Reaction Size
10031246 ddPCR MUT FAM Assay, Validated 200
10031247 ddPCR MUT FAM Assay, Validated 1000
10031248 ddPCR MUT FAM Assay, Validated 2500
10031249 ddPCR MUT HEX Assay, Validated 200
10031250 ddPCR MUT HEX Assay, Validated 1000
10031251 ddPCR MUT HEX Assay, Validated 2500
10042964 ddPCR MUT FAM Assay 200R 200
10042965 ddPCR MUT FAM Assay 1000R 1000
10042966 ddPCR MUT FAM Assay 2500R 2500
10042967 ddPCR MUT HEX Assay 200R 200
10042968 ddPCR MUT HEX Assay 1000R 1000
10042969 ddPCR MUT HEX Assay 2500R 2500
12008220 ddPCR MUT Cy5 Assay 200
12008221 ddPCR MUT Cy5 Assay 1000
12008222 ddPCR MUT Cy5.5 Assay 2500
12008223 ddPCR MUT Cy5.5 Assay 200
12008224 ddPCR MUT Cy5.5 Assay 1000
12008225 ddPCR MUT Cy5.5 Assay 2500

Related Products

Resources

Documents

Documents

Number Description Options
7144
Wet-Lab Validated ddPCR Assays for Mutation Detection and Copy Number Determination Brochure
10000050420
ddPCR Mutation Detection Assays, Validated, Product Insert
10000050422
ddPCR Mutation Detection Assays Product Insert
10033487
ddPCR Mutation Detection Assays, Validated, Product Insert
10047489
ddPCR Mutation Detection Assays Product Insert