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ddPCR Mutation detection assays can be found and purchased on Bio-Rad.com/digital-assays. A wet-lab validated mutation detection assay is returned if available, or an assay is designed instantly for your target. Wet-lab validated mutation detection assays detect mutant DNA present at 0.1% in a background of WT DNA in one well. An even better sensitivity of 0.005–0.1% can generally be achieved by using multiple reaction wells. Browse list of wet-lab validated Mutation Detection Assays.
Mutation detection assays can be designed instantly for over 4 million mutations, listed in the COSMIC database, on Bio-Rad’s Digital Assay Site. Built by the experts in digital PCR at Bio-Rad's Digital Biology Center, the Bio-Rad Digital Assay Site design engine takes into consideration everything from GC content and Tm, to primer placement and probe length, even genome specificity when designing an assay for you.
- Wet-lab validated assays available for more than 200 targets
- Wet-lab validated assays guaranteed to work when used as instructed
- Validation data available for review and download
- Assays designed instantly for targets and sequences for which validated assays are not available
- Suitable for use with circulating tumor DNA, liquid biopsy, and FFPE and other tissue samples
- Orderable in single-tube or two-tube formats
- Positive control sequences provided
High sensitivity of PrimePCR ddPCR mutation detection assays provide precise quantification of JAK2 V617F mutation in the presence of wild-type DNA. A, 2-D fluorescence amplitude plot shows duplicate wells of a mixed mutant: wild-type sample across multiple percentage mutant blends. The black cluster on the plot represents the negative droplets, the green cluster represents the droplets that are positive for wild-type DNA only, the blue cluster represents the droplets that are positive for mutant DNA only, and the orange cluster represents the droplets that are positive for both wild-type and mutant targets. B, 2-D fluorescence amplitude plot shows duplicate wells of a wild-type–only sample. C, fractional abundance plot shows the percentage frequency (orange markers) of the mutant DNA in a wild-type DNA background. All error bars are generated by QuantaSoft software and represent the 95% confidence interval.
ddPCR Mutation Detection Assays are available in single tube or two-tube formats.
The single-tube formats of the ddPCR Mutation Detection Assays contain a set of primers, a mutant, and wild-type probe. The single tube format assays are 20X concentrated, ready-to-use-primer-probe mix optimized for use with ddPCR Supermix for Probes (no dUTP). Each assay contains 18 uM each primer and 5uM each probe. The single-tube formats provide added convenience as the mutant and WT probes are pre-mixed and allow for the addition of an extra sample in the ddPCR reaction.
|Catalog#||Product Name||Reaction Size|
|10049550||ddPCR MUT FAM+HEX Assay, Validated||200|
|10049551||ddPCR MUT FAM+HEX Assay, Validated||1000|
|10049552||ddPCR MUT FAM+HEX Assay, Validated||2500|
|10049047||ddPCR MUT FAM+HEX Assay||200|
|10049048||ddPCR MUT FAM+HEX Assay||1000|
|10049049||ddPCR MUT FAM+HEX Assay||2500|
|12005581||ddPCR MUT Cy5+Cy5.5 Assay||200|
|12005580||ddPCR MUT Cy5+Cy5.5 Assay||1000|
|12005579||ddPCR MUT Cy5+Cy5.5 Assay||2500|
The mutant and WT probes are provided separately.
|Catalog#||Product Name||Reaction Size|
|10031246||ddPCR MUT FAM Assay, Validated||200|
|10031247||ddPCR MUT FAM Assay, Validated||1000|
|10031248||ddPCR MUT FAM Assay, Validated||2500|
|10031249||ddPCR MUT HEX Assay, Validated||200|
|10031250||ddPCR MUT HEX Assay, Validated||1000|
|10031251||ddPCR MUT HEX Assay, Validated||2500|
|10042964||ddPCR MUT FAM Assay 200R||200|
|10042965||ddPCR MUT FAM Assay 1000R||1000|
|10042966||ddPCR MUT FAM Assay 2500R||2500|
|10042967||ddPCR MUT HEX Assay 200R||200|
|10042968||ddPCR MUT HEX Assay 1000R||1000|
|10042969||ddPCR MUT HEX Assay 2500R||2500|
|12008220||ddPCR MUT Cy5 Assay||200|
|12008221||ddPCR MUT Cy5 Assay||1000|
|12008222||ddPCR MUT Cy5.5 Assay||2500|
|12008223||ddPCR MUT Cy5.5 Assay||200|
|12008224||ddPCR MUT Cy5.5 Assay||1000|
|12008225||ddPCR MUT Cy5.5 Assay||2500|