Absolute Answers,
One Droplet at a Time

Session: Clinical Utility of ddPCR for Detecting POLE Mutations in Endometrial Cancer.

This presentation will explore the clinical utility of Droplet Digital PCR (ddPCR) for detecting POLE mutations in endometrial cancer. Dr. Hong will discuss the advantages of ddPCR for highly sensitive mutation detection and its potential role in molecular classification, prognosis, and precision treatment strategies for patients with endometrial cancer.

Session: Droplet-Level Precision: Redefining Sensitivity in Oncology.

In this session, Dr. Salil Vaniawala will discuss how droplet digital PCR is redefining sensitivity in oncology through precise and rapid detection of cancer biomarkers. The presentation will highlight how ddPCR technology enables absolute quantification of low variant allele frequency mutations across liquid biopsy, minimal residual disease monitoring, and precision oncology applications.

Session: Droplet Digital PCR for NPM1 Measurable Residual Disease Monitoring in AML.

Measurable residual disease (MRD) assessment is integral to risk stratification and treatment monitoring in acute myeloid leukemia (AML), with NPM1 mutations serving as a robust molecular marker for disease surveillance. This presentation evaluates the utility of Droplet Digital PCR (ddPCR) as a highly sensitive and precise platform for NPM1 MRD tracking. The local prevalence and molecular epidemiology of NPM1 mutation subtypes within a Singapore cohort will be outlined.

Next, analytical validation data of ddPCR assays specifically engineered for the common NPM1 variants (Types A, B, and D) will be presented. Ongoing developmental strategies to extend MRD monitoring capabilities to patients harboring rare NPM1 variants will also be highlighted. This seminar illustrates how synthesizing local epidemiological data, robust assay validation, and clinical implementation can establish a comprehensive, future-ready ddPCR MRD program to optimize AML patient management.

Session: Digital PCR Meets MSRE: Detecting DNA Methylation in Cell-free DNA from Breast Cancer Patients

In this presentation, Dr. Tey will discuss the integration of methylation-sensitive restriction enzyme (MSRE) digestion into a droplet digital PCR workflow for the detection of tumor-derived methylation signals in cell-free DNA from breast cancer patients. He will share key insights into assay design, analytical validation, pilot study findings, and future development strategies aimed at improving the accuracy and clinical applicability of early breast cancer screening.

Session: Decoding Telomere Dynamics in Cellular Senescence: A Droplet Digital PCR Approach from Telomerase Activity to TERT Isoform Landscape

This presentation will highlight the application of droplet digital PCR (ddPCR) to investigate telomere biology in cellular senescence. Dr. Kim will present how ddPCR enables precise analysis of telomerase activity and TERT isoform expression, providing valuable insights into cellular aging and the development of age-related diseases.

Session: Extracellular Vesicle DNA as a Next-Generation Liquid Biopsy: Clinical Applications Through Droplet Digital PCR

In this session, Dr. Yokoi will discuss the role of extracellular vesicles, including exosomes, as promising sources for liquid biopsy. Because EVs circulate in body fluids and protect their molecular cargo from degradation, EV-derived DNA has gained increasing attention for its ability to reflect the genomic landscape of originating cells.

Session: Establishing ddPCR-based Minimal Molecular Quality Control Criteria for Therapeutic Extracellular Vesicles

Extracellular vesicles (EVs), including exosomes, are emerging as promising therapeutic agents due to their natural role in cell-to-cell communication and ability to deliver functional biomolecules. Ensuring consistent quality during EV production is critical, requiring sensitive and reliable molecular quality control (QC) methods.

Droplet digital PCR (ddPCR) enables precise and absolute quantification of nucleic acid cargo, making it a powerful tool for establishing minimal molecular QC criteria and ensuring batch-to-batch consistency. Please join us in this session to learn more about the QC process and how ddPCR can support robust quality control for therapeutic extracellular vesicles.

Session: Application of ddPCR based RCL testing for FKC289-a product of Fosunova-T platform.

CAR-T therapies require rapid, reliable manufacturing and rigorous quality control to ensure product safety and efficacy. The Fosunova-T platform enables closed, automated production of FKC289, a BCMA/CD19 dual-target CAR-T product, within 2–3 days. To support rapid product release, a ddPCR-based linkage assay was developed for sensitive and specific detection of replication-competent lentivirus (RCL), minimizing false-positive results caused by residual plasmid DNA. Please join us to learn how ddPCR can enable robust RCL testing and accelerate quality control for next-generation CAR-T manufacturing.

Session: Precision Without Compromise: the Role of Droplet Digital PCR in De-Risking Biotherapeutic Development

Advanced biomanufacturing requires molecular data that is fast, absolute, and reproducible, yet conventional methods such as qPCR and protein assays can introduce variability or delay critical decisions. This session will explore how Droplet Digital PCR (ddPCR) helps address these challenges across key biomanufacturing workflows, including cell line development for bispecific antibodies and AAV vector characterization.

Drawing on a peer-reviewed case study with Boehringer Ingelheim, the session will highlight how ddPCR enables absolute quantification of transgene copy number and expression, supporting earlier and more efficient clone screening. It will also cover how multiplexed ddPCR, linkage analysis, and the VeriCheck ddPCR Empty-Full Capsid Kit can support viral vector characterization. Together, these examples demonstrate how ddPCR provides the precision, sensitivity, and reproducibility needed to accelerate decision-making and de-risk biotherapeutic development programs.

Session: SinChro: A Novel Method to Count Chromosomal Numbers at Single Cell Level, Applied in Aging Research

In this session, Dr. Tomohiro Kubo will introduce SinChro, a novel technology that applies droplet digital PCR to measure chromosome copy number at the single-cell level. The method uses fluorescent labeling within droplets to quantify the number of copies of a specific chromosome in individual cells.

Session: Testing and manufacturing industry standards using ddPCR

Digital droplet PCR (ddPCR) has become a critical technology for the development, characterisation, and quality assurance of DNA measurements. Its ability to provide precise, sensitive, and calibration-independent quantification makes it particularly valuable for supporting manufacturing processes, product validation, and measurement traceability across the biotechnology, diagnostics, and life sciences sectors.

This presentation will highlight the role of ddPCR in the development and certification of reference materials that are traceable to the International System of Units (SI). It will showcase the work undertaken by the National Measurement Institute (NMI) in producing highly characterised DNA and RNA reference materials that support reliable and comparable molecular measurements. The presentation will demonstrate how ddPCR, together with isotope dilution mass spectrometry (IDMS), underpins robust manufacturing processes, measurement traceability, and confidence in molecular testing results. It will also emphasise the importance of global standardisation of genetic measurements to ensure consistency, comparability, and regulatory alignment across laboratories and countries.