Using Droplet Digital PCR for Cancer and Liquid Biopsy Studies


Droplet Digital PCR (ddPCR) technology is being used to identify cancer subtypes, optimize drug treatment plans, monitor residual disease, and study tumor evolution. ddPCR assays offer advantages in the field of liquid biopsies, enabling the measurement of circulating nucleic acids (cfDNA) and circulating tumor cells (CTCs) in blood. ddPCR can detect rare tumorigenic mutations in a high background of normal DNA, routinely down to 0.01% and often further. Dr. Dawne Shelton discusses the techniques and methods that will enable you to begin your investigations.