qPCR Unvalidated:
What Your Data Isn't Telling You
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Relying solely on Cq values to judge qPCR performance can be misleading — low Cq may indicate non-specific amplification, poor efficiency, or PCR inhibitors. Proper validation requires multiple evaluation methods to ensure specificity, efficiency, and reproducibility.
Join Bio-Rad Field Application Scientists in this free webinar as they break down key steps for qPCR assay validation, from reagent selection to standard and melt curve analysis. Learn when Droplet Digital PCR (ddPCR) technology may be a better alternative for gene expression studies.
Key Takeaways:
- Why Cq values can be misleading
- Best practices for qPCR assay validation
- Troubleshooting non-specific amplification
- When to use ddPCR over qPCR
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Speakers
David Eaves
Field Application Scientist
David Eaves is a molecular biology expert with over 15 years of experience in research and clinical settings. He has applied this expertise to support scientists as a Field Application Scientist and Technical Sales Specialist at Bio-Rad Laboratories. Previously, he spent eight years at Cincinnati Children's Hospital Medical Center as a Senior Molecular Pathology Technician, specializing in clinical sequencing applications.
Jonathan Rangel, Ph.D.
Field Application Scientist
Jonathan is a Genomics Field Application Scientist at Bio-Rad Laboratories, supporting researchers across the South-Central United States. He earned his Ph.D. in Biochemistry from the University of Houston, where he studied bacterial stress response systems using PCR/qPCR, protein characterization, and microbiology techniques. With nine years of experience as a scientist and three years at Bio-Rad, he specializes in real-time PCR and droplet digital PCR, providing technical support and guidance to researchers using these genomic technologies in their labs.
Katie Orban, Ph.D.
Field Application Scientist
Katie is a genomics Field Application Scientist at Bio-Rad Laboratories, supporting biopharma researchers in southern California. She received her Ph.D. in molecular biology from the University of Southern California, where her research focused on bacterial DNA structure and long-term population dynamics using molecular biology and microbiology techniques. Her genomics experience lends itself to her current role at Bio-Rad, where she specializes in qPCR and ddPCR.
Sanket Joshi, Ph.D.
Field Application Team Leader, Pacific
Sanket is a Field Application Team Leader at Bio-Rad Pacific. Prior to joining the commercial sector, he completed a Ph.D. in Cancer Biology from the University of Sydney, followed by post-doctoral studies at Cancer Research UK and the University of New South Wales. Sanket is passionate about helping customers in research and pathology labs and provide application and technical support to get the most out of Bio-Rad’s products.