September 3, 2009
Dr. Sharon Willis
Head of Lipoparticle Production
Integral Molecular, Inc
Abstract:
In this webinar, Dr Sharon Willis, cofounder and head of lipoparticle production at Integral Molecular, Inc., discusses the use of lipoparticles and the ProteOn protein interaction array system in her work investigating membrane protein interactions. Dr Willis shares insights gained in over 12 years of work in biomolecular interaction studies and expertise in viral lipoprotein structure. She relates some of the difficulties involved in the study of membrane-bound proteins outside of the cell membrane and the novel use of non-infectious virion cores to create synthetic lipoparticles bearing intact cell membrane proteins of interest. This method preserves the native structures of membrane proteins and presents several advantages over cell membrane preps for subsequent high-throughput screening in a microfluidic system. Dr Willis presents the development of antibody binding assays in which lipoparticles are captured on the ProteOn chip surface to determine the kinetics of antibody interactions with lipoparticle-bound cell-surface receptors.
About the speaker:
Dan Fox is Director of Research and Development at Propel Labs, in Fort Collins, Colorado. Dan has over a decade of experience in designing high-speed cell sorters, and holds numerous patents on flow cytometry and sorting technology. He is one of the principal designers who developed Bio-Rad’s S3 Cell Sorter.