Droplet Digital™ PCR Assays: How to Design a Multiplex Experiment with EvaGreen

May 12, 2015
Sonali Mahalanabis, PhD
Technical Support Consultant
Bio-Rad Life Science Research
22.31 min

Abstract:
Sonali Mahalanabis from Bio-Rad Tech Support discusses the advantages of multiplexing Droplet Digital PCR (ddPCR™) assays with EvaGreen® dye.

The Droplet Digital PCR system allows for the quantification of target DNA with unrivaled precision by partitioning samples into 20,000 nanoliter-sized droplets. After PCR on a thermal cycler, PCR-positive and PCR-negative droplets are counted to provide absolute quantification of the target DNA.