Droplet Digital™ PCR Applications for Next-Generation Sequencing

Droplet Digital™ PCR Applications for Next Generation Sequencing

January 7, 2014
Nicholas Heredia, PhD
Staff Scientist, Bio-Rad Digital Biology Center


Next-generation sequencing (NGS) platforms are currently being used to quantify rare species targets in samples. Heavy processing of the starting template in the NGS experimental workflow can result in biases in the representation of these targets.

In this webinar, we discuss Droplet Digital PCR (ddPCR™) applications for cross-validating NGS data with respect to rare species targets as well as the excellent capability of ddPCR quantification of NGS libraries to optimize sequencing performance.

The QX200™ Droplet Digital PCR system, Bio-Rad's second-generation digital PCR system, provides absolute quantification of target DNA or RNA molecules using EvaGreen or TaqMan hydrolysis probes, yielding unmatched sensitivity and precision for a wide variety of applications.

About the speaker:
Dr. Heredia received his PhD in microbiology, immunology, and molecular genetics at the University of California, Los Angeles and performed postdoctoral studies in biophysics at the Lawrence Livermore National Laboratory. At the Digital Biology Center at Bio-Rad Laboratories, he is focusing on digital applications for NGS as well as advanced R&D research.