The application of ddPCR for SARS‑CoV‑2 detection in low viral load samples
Prof Yu Chen, PhD
State Key Laboratory of Virology, College of Life Science, WuHan University
Dr Chen is engaged in the research of replication and pathogenesis of medical viruses such as coronavirus and virus control, analyzing the functions and structures of SARS coronavirus RNA methyltransferase, main protease and N protein and their mechanisms of action, and developed four targeted anti-coronavirus inhibitors. During the SARS‑CoV‑2 outbreak, he was part of the investigation team to complete the pathogen identification and the whole genome sequencing on January 7, 2020. Subsequently, Dr Chen identified the aerosol novel coronavirus load and transmission characteristics by developing a highly sensitive nucleic acid detection method based on Droplet Digital PCR. The studies show ddPCR could largely minimize the false negatives reports resulted by qRT‑PCR. Dr Chen has published more than 40 papers with more than 4800 citations in Nature, Molecular Cell, Nature Immunology, Nature Ecology & Evolution, PNAS, PLoS Pathogens, Emerging Microbes & Infections, Journal of Virology.
About the speaker:
Prof Yu Chen, PhD
State Key Laboratory of Virology, College of Life Science, WuHan University
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