Hercules, CA — May 6, 2015 — Biogazelle, a qPCR and RNA sequencing (RNA-seq) data analysis and services company, recently used Bio-Rad Laboratories’ PrimePCR Real-Time PCR Assays in a global analysis of next-generation sequencing (NGS) reproducibility that was published in Nature Biotechnology.
This FDA-led Sequencing Quality Control (SEQC) study compared RNA-seq data generated using different NGS platforms to benchmark their performance. The authors compared different NGS platforms and how they performed on well-characterized RNA samples. They also compared those data to microarray and qPCR data.
Biogazelle used almost 21,000 PrimePCR Assays to assess the expression levels of protein-coding genes in four samples from MAQC-I, a previous FDA-led study to compare different microarray technologies. In total, Biogazelle performed more than 80,000 qPCR reactions. These reference data matched well with RNA-seq results.
“Our motivation to participate in the SEQC study was to add a valuable reference for evaluating the results generated by RNA sequencing,” said Jan Hellemans, CEO and cofounder of Biogazelle. “Bio-Rad’s human PrimePCR Assays are fully wet-lab validated, making them ideally suited for benchmarking.”
Bio-Rad and Biogazelle partnered to develop PrimePCR Assays in 2012, seeking to achieve a level of validation that goes beyond industry standards and common research practices. The raw validation data that come with each assay ensure studies are predictable and controlled. Researchers can also use PrimePCR Assays to follow qPCR best practices known as MIQE (minimum information for publication of quantitative real-time PCR experiments).
For more information about Bio-Rad’s PrimePCR Assays, please visit www.bio-rad.com/PrimePCRpr.
Bio-Rad Laboratories, Inc. (NYSE: BIO and BIOb) develops, manufactures, and markets a broad range of innovative products and solutions for the life science research and clinical diagnostic markets. The company is renowned for its commitment to quality and customer service among university and research institutions, hospitals, public health and commercial laboratories, as well as the biotechnology, pharmaceutical, and food safety industries. Founded in 1952, Bio-Rad is based in Hercules, California, and serves more than 100,000 research and healthcare industry customers through its global network of operations. The company employs more than 7,600 people worldwide and had revenues exceeding $2.1 billion in 2014. For more information, visit our website at www.bio-rad.com.
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