Fixable Viability Dyes

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Overview

Available Fixable Viability Dyes

VivaFix™ Cell Viability Assays Excitation Maximum, nm Emission Maximum, nm Optimal Excitation Laser, nm Optimal Filter Set for S3™ 488/561 nm Cell Sorter Catalog Number
VivaFix 353/442 353 442 355 135-1111
VivaFix 410/450 410 450 405 135-1112
VivaFix 408/512 408 512 405 135-1113
VivaFix 398/550 398 550 405 135-1114
VivaFix 498/521 498 521 488 FL1 (525/30) 135-1115
VivaFix 547/573 547 573 561 FL2 (586/25) 135-1116
VivaFix 583/603 583 603 561 FL3 (615/25) 135-1117
VivaFix 649/660 649 660 640 135-1118

 

VivaFix™ Wavelength Spectra

Excitation Wavelength Spectrum
VivaFix Excitation Wavelength Spectrum

Emission Wavelength Spectrum
VivaFix Emission Wavelength Spectrum

Flow Cytometry Performance Data

Excellent separation between live and dead cells

Flow Cytometry Performance Data

Jurkat cells were stained with the VivaFix 498/521 cell viability assay, fixed with 3.7% formaldehyde (A) or not fixed (B), and analyzed with the S3™ cell sorter. SSC, side scatter.

Fluorescence Microscopy Performance Data
Bright field
Fluorescence
channel
  Dead Live + dead

Visualization of MCF-7 cells analyzed by fluorescence microscopy. The cells were stained with VivaFix™ 498/521 cell viability assay and fixed with 3.7% formaldehyde. The arrow indicates a dead cell whose greater fluorescence intensity clearly distinguishes it from the cluster of live cells that surrounds it.

The VivaFix™ cell viability assays assist researchers with accurately discriminating between live and dead cells in fixed or unfixed samples by flow cytometry or cell sorting.

The proprietary components of the VivaFix fixable viability dyes covalently bind to free primary amines: in live cells, these fixable viability dyes bind to the cell surface primary amines. In dead cells, where the membrane is compromised, the dyes are able to permeate the cells and also react with intracellular primary amines (Figure 1). Due to the greater amount of accessible free amines, the dying cells acquire a brighter fluorescence compared to the live cells, allowing the live and dead cell populations to be easily distinguished.


Fig. 1. VivaFix cell viability assay chemistry. A, live cell with VivaFix dyes bound to surface primary amines. B, dead cell with VivaFix dyes bound to surface and intracellular primary amines.

Key Benefits of Fixable Viability Dyes

  • Compatible with fixed and unfixed cells
  • Provides unbiased discrimination between live and dead cell populations — at least 100-fold difference in fluorescence intensity
  • Offered in a wide range of excitation and emission spectra to fit any multicolor experiment

Related Products

The following DNA-binding cell viability assays are also available to assess the health of your cells of interest:

Additional Products for Flow Cytometry

Bio-Rad also offers reagents and consumables to complete each step of flow cytometry experiments:

  • Cell proliferation assays — easily incorporate 1 of 5 cell proliferation dyes into your multicolor experiment to monitor cell health or cell division
  • Antibody labeling kits — label your antibody of interest in 2 easy steps without a purification column
  • Primary antibodies — use high-quality monoclonal and polyclonal primary antibodies for applications ranging from stem cell, immunology, and cell biology to veterinary research

More Information

Learn more about the features of the S3™ cell sorter and its associated consumables and accessories.

VivaFix™ 353/442 Cell Viability Assay
VivaFix™ 353/442 Cell Viability Assay

135-1111
Pkg of 1, kit includes 4 x 50 assay vials and 250 µl of DMSO, for running 200 assays

List Price:   $250.00
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VivaFix™ 410/450 Cell Viability Assay
VivaFix™ 410/450 Cell Viability Assay

135-1112
Pkg of 1, kit includes 4 x 50 assay vials and 250 µl of DMSO, for running 200 assays

List Price:   $250.00
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VivaFix™ 408/512 Cell Viability Assay
VivaFix™ 408/512 Cell Viability Assay

135-1113
Pkg of 1, kit includes 4 x 50 assay vials and 250 µl of DMSO, for running 200 assays

List Price:   $250.00
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VivaFix™ 398/550 Cell Viability Assay
VivaFix™ 398/550 Cell Viability Assay

135-1114
Pkg of 1, kit includes 4 x 50 assay vials and 250 µl of DMSO, for running 200 assays

List Price:   $250.00
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VivaFix™ 498/521 Cell Viability Assay
VivaFix™ 498/521 Cell Viability Assay

135-1115
Pkg of 1, kit includes 4 x 50 assay vials and 250 µl of DMSO, for running 200 assays

List Price:   $250.00
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VivaFix™ 547/573 Cell Viability Assay
VivaFix™ 547/573 Cell Viability Assay

135-1116
Pkg of 1, kit includes 4 x 50 assay vials and 250 µl of DMSO, for running 200 assays

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VivaFix™ 583/603 Cell Viability Assay
VivaFix™ 583/603 Cell Viability Assay

135-1117
Pkg of 1, kit includes 4 x 50 assay vials and 250 µl of DMSO, for running 200 assays

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VivaFix™ 649/660 Cell Viability Assay
VivaFix™ 649/660 Cell Viability Assay

135-1118
Pkg of 1, kit includes 4 x 50 assay vials and 250 µl of DMSO, for running 200 assays

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Number Description Options
10041645 VivaFix™ Cell Viability Assay Product Insert, Rev A Click to download
6559 VivaFix™ Cell Viability Assays Flier, Rev A Click to download