7-AAD Solution

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7-AAD Solution


The ReadiDrop™ 7-aminoactinomycin D (7-AAD) cell viability assay is designed as a ready-to-use 7-AAD solution, which binds double-stranded DNA with a high-affinity intercalation between GC base pairs.

The assay removes the traditional manual weighing, pipetting, and dilution steps when using commonly available cell viability dyes in flow cytometry experiments. Simply add 1 or 2 drops of the cell viability assay to assess the health of your cells.

Key Benefits of the ReadiDrop 7-AAD Cell Viability Dye

  • Ideal for cell sorting — fluorescent molecules are suspended in PBS without preservatives
  • Stable at room temperature — keep a bottle of ReadiDrop 7-AAD cell viability assay by your cell sorter or cell analyzer for easy access
  • No more weighing, pipetting, and diluting — ready-to-use solution gives 1 µg/ml as the final concentration

Features of the ReadiDrop 7-AAD Cell Viability Dye

  • Fluorophore Properties
    • Excitation maximum: 548 nm
    • Emission maximum: 648 nm
    • Optimal excitation laser: 561 nm or 488 nm
  • Fluorescent Spectrum

 Fluorescent Spectrum

  • Molecular weight: 1270.43 g/mol
  • Molecular formula: C27H34I2N4
  • Chemical structure

Chemical Structure

  • Specifications
    • Store at 15–30°C
    • Do not freeze

More Information

Learn more about the features of the S3e™ Cell Sorter and its associated consumables.

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ReadiDrop™ 7-AAD

1351102
9 ml, (3 x 3 ml) ready-to-use dropper bottles for dead cell exclusion

1351102 /templatedata/internet/product_SKU/data/LSR/Flow-Cytometry/Cell-Viability-Dyes/135-1102_1380151792.xml 1351102 /default/main/bio-rad/WORKAREA/default 09/25/13 04:21 PM en_US jebrbicf 2018-02-28 05:09:16 true true internet/product_SKU jebrbicf templatedata/internet/product_SKU/data/LSR/Flow-Cytometry/Cell-Viability-Dyes/135-1102_1380151792.xml LSR 2018-03-06 01:41:39 /default/main/bio-rad/STAGING N /default/main/bio-rad en 09/25/23 04:22 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2018-03-06 01:41:39 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Flow-Cytometry/Cell-Viability-Dyes/135-1102_1380151792.xml templatedata/internet/product_SKU/data/LSR/Flow-Cytometry/Cell-Viability-Dyes/135-1102_1380151792.xml 1351102-readidrop-7-aad 1351102 ReadiDrop 7-AAD ReadiDrop&trade; 7-AAD 9 ml, (3 x 3 ml) ready-to-use dropper bottles for dead cell exclusion /webroot/web/images/lsr/products/flow_cytometry/sku_thumb/global/readidrop-cell-viability-assays-135-1102-thumb.jpg /webroot/web/images/lsr/products/flow_cytometry/sku_view/global/readidrop-cell-viability-assays-135-1102-view.jpg preview Eddie C ReadiDrop 7-AAD 9 ml, (3 x 3 ml) ready-to-use dropper bottles for dead cell exclusion 1351102, readidrop, 7-aad, 7-aminoactinomycin d, ready-to-use, cell, viability, dye, exclude, exclusion, dead, cells, flow, cytometry 09/25/13 04:21 PM 09/25/23 04:22 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Flow-Cytometry/Cell-Viability-Dyes N 0 <p>Use <strong>ReadiDrop 7-AAD</strong> (7-aminoactinomycin D), a ready-to-use cell viability dye, for excluding dead cells in flow cytometric experiments. As a cell-impermeable assay, ReadiDrop 7-AAD enters a compromised cell and binds to double-stranded DNA with a high-affinity intercalation between GC base pairs.</p> <p>The assay removes the traditional manual weighing, pipetting, and dilution steps when using commonly available cell viability dyes in flow cytometry experiments. Simply add 1 or 2 drops of the cell viability assay to assess the health of your cells.<br /></p> <p><img src="/webroot/web/images/lsr/products/flow_cytometry/product_detail/global/7aad-solution-graph-content.jpg" alt="" /></p> <h2 class="descHeader">Key Benefits of the ReadiDrop 7-AAD Cell Viability Dye</h2> <ul> <li><strong>Ideal for cell sorting</strong> &mdash; fluorescent molecules are suspended in PBS without preservatives</li> <li><strong>Stable at room temperature</strong> &mdash; keep a bottle of any ReadiDrop 7-AAD cell viability assay by your cell sorter or cell analyzer for easy access</li> <li><strong>No more weighing, pipetting, and diluting</strong> &mdash; ready-to-use solution gives 1 &micro;g/ml as the final concentration </li> </ul> <h2 class="descHeader">Features of the ReadiDrop 7-AAD Cell Viability Dye</h2> <ul> <li><strong>Fluorophore Properties</strong> <ul> <li>Excitation maximum: 548 nm</li> <li>Emission maximum: 648 nm</li> <li>Optimal excitation laser: 561 nm or 488 nm</li> </ul> </li> </ul> <ul> <li><strong>Fluorescent Spectrum</strong></li> </ul> <p>&nbsp;<img src="/webroot/web/images/lsr/products/flow_cytometry/product_detail/global/fluorescent-spectrum-135-1102a.gif" alt="Fluorescent Spectrum" /></p> <ul> <li><strong>Molecular weight</strong>: 1270.43 g/mol</li> <li><strong>Molecular formula</strong>: C<sub>62</sub>H<sub>87</sub>N<sub>13</sub>O<sub>16</sub></li> <li><strong>Chemical structure</strong></li> </ul> <p><img src="/webroot/web/images/lsr/products/flow_cytometry/product_detail/global/chemical-structure-135-1102b.gif" alt="Chemical Structure" /><br /></p> <ul> <li><strong>Specifications</strong> <ul> <li>Store at 15&ndash;30&deg;C</li> <li>Do not freeze</li> </ul> </li> </ul> <h2 class="descHeader">Related Products</h2> <p>Design your custom flow cytometric experiment with our available fixable viability dyes:</p> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr class="pd_tableheader"> <td valign="bottom"><strong>VivaFix&trade; Cell Viability Assays </strong></td> <td align="center" valign="bottom"><strong>Excitation Maximum, nm</strong></td> <td align="center" valign="bottom"><strong>Emission Maximum, nm</strong></td> <td align="center" valign="bottom"><strong>Optimal Excitation Laser, nm</strong></td> <td align="center" valign="bottom"><strong>Optimal Filter Set for S3&trade; 488/561 nm Cell Sorter</strong></td> <td align="center" valign="bottom"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td valign="top">VivaFix 353/442</td> <td align="center" valign="top">353</td> <td align="center" valign="top">442</td> <td align="center" valign="top">355</td> <td align="center" valign="top">&ndash;</td> <td align="center" valign="top"><a style="font-size:11px" href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=135-1111">135-1111</a></td> </tr> <tr> <td valign="top">VivaFix 410/450</td> <td align="center" valign="top">410</td> <td align="center" valign="top">450</td> <td align="center" valign="top">405</td> <td align="center" valign="top">&ndash;</td> <td align="center" valign="top"><a style="font-size:11px" href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=135-1112">135-1112</a></td> </tr> <tr class="pd_colorbackground"> <td valign="top">VivaFix 408/512</td> <td align="center" valign="top">408</td> <td align="center" valign="top">512</td> <td align="center" valign="top">405</td> <td align="center" valign="top">&ndash;</td> <td align="center" valign="top"><a style="font-size:11px" href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=135-1113">135-1113</a></td> </tr> <tr> <td valign="top">VivaFix 398/550</td> <td align="center" valign="top">398</td> <td align="center" valign="top">550</td> <td align="center" valign="top">405</td> <td align="center" valign="top">&ndash;</td> <td align="center" valign="top"><a style="font-size:11px" href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=135-1114">135-1114</a></td> </tr> <tr class="pd_colorbackground"> <td valign="top">VivaFix 498/521</td> <td align="center" valign="top">498</td> <td align="center" valign="top">521</td> <td align="center" valign="top">488</td> <td align="center" valign="top">FL1 (525/30)</td> <td align="center" valign="top"><a style="font-size:11px" href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=135-1115">135-1115</a></td> </tr> <tr> <td valign="top">VivaFix 547/573</td> <td align="center" valign="top">547</td> <td align="center" valign="top">573</td> <td align="center" valign="top">561</td> <td align="center" valign="top">FL2 (586/25)</td> <td align="center" valign="top"><a style="font-size:11px" href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=135-1116">135-1116</a></td> </tr> <tr class="pd_colorbackground"> <td valign="top">VivaFix 583/603</td> <td align="center" valign="top">583</td> <td align="center" valign="top">603</td> <td align="center" valign="top">561</td> <td align="center" valign="top">FL3 (615/25)</td> <td align="center" valign="top"><a style="font-size:11px" href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=135-1117">135-1117</a></td> </tr> <tr> <td valign="top">VivaFix 649/660</td> <td align="center" valign="top">649</td> <td align="center" valign="top">660</td> <td align="center" valign="top">640</td> <td align="center" valign="top">&ndash;</td> <td align="center" valign="top"><a style="font-size:11px" href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=135-1118">135-1118</a></td> </tr> <tr> <td style="border-right: 0; border-left: 1px solid #fff" colspan="6" valign="bottom">&nbsp;</td> </tr> <tr class="pd_tableheader"> <td valign="bottom"><strong>ReadiDrop Cell Viability Assays</strong></td> <td align="center" valign="bottom"><strong>Excitation Maximum, nm</strong></td> <td align="center" valign="bottom"><strong>Emission Maximum, nm</strong></td> <td align="center" valign="bottom"><strong>Optimal Excitation Laser, nm</strong></td> <td align="center" valign="bottom"><strong>Optimal Filter Set for S3 488/561 nm Cell Sorter </strong></td> <td align="center" valign="bottom"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td valign="top">ReadiDrop propidium iodide</td> <td align="center" valign="top">538</td> <td align="center" valign="top">617</td> <td align="center" valign="top">488</td> <td align="center" valign="top">FL3 (585/25)</td> <td align="center" valign="top"><a style="font-size:11px" href="/en-us/sku/1351101-readidrop-propidium-iodide?ID=1351101">135-1101</a></td> </tr> <tr> <td valign="top">ReadiDrop 7-AAD</td> <td align="center" valign="top">548</td> <td align="center" valign="top">648</td> <td align="center" valign="top">488</td> <td align="center" valign="top">FL4 (655LP)</td> <td align="center" valign="top">135-1102</td> </tr> </tbody> </table> <br /> <h2 class="descHeader">Additional Products for Flow Cytometry</h2> <p>Bio-Rad also offers reagents and consumables to complete each step of flow cytometry experiments:</p> <ul> <li><a href="/en-us/product/propidium-iodide-solution">Propidium iodide solution</a> &mdash; assess cell health with no weighing, pipetting, or dilution steps </li> <li><a href="/evportal/destination/commerce/product_detail?catID=MTFV5T4VY">Cell proliferation assays</a> &mdash; easily incorporate 1 of 5 cell proliferation dyes into your multicolor experiment to monitor cell health or cell division</li> <li><a href="/evportal/destination/commerce/product_detail?catID=MTFV3M15">Antibody labeling kits</a> &mdash; label your antibody of interest in 2 easy steps without a purification column</li> <li><a href="http://www.abdserotec.com/primary-antibodies-reagents.html">Primary antibodies</a> &mdash; use high-quality monoclonal and polyclonal primary antibodies for applications ranging from stem cell, immunology, and cell biology to veterinary research</li> <li><a href="/en-us/product/fixable-viability-dyes">Fixable viability dyes</a> &mdash; easily introduce a viability assay in your multicolor experiments </li> </ul> <h2 class="descHeader">More Information</h2> <p>Learn more about <a href="/en-us/product/7-aad-solution">ReadiDrop&trade; 7-AAD solution</a> &nbsp;and the <a href="/en-us/product/cell-sorting/s3e-cell-sorter?ID=OK1GROIVK">features of the S3e&trade; Cell Sorter</a>.</p> /webroot/web/html/lsr/products/flow_cytometry/sku_view/description/global readiDrop-7-aad.html /webroot/web/html/lsr/products/flow_cytometry/sku_view/description/global/readiDrop-7-aad.html 1 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1351102&CartQuote=QT&quantity= 1107 false true false $278.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1351102&AddToDefaultWishList=true&quantity= true false true 12171500 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1351102&quantity= 1351102
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6522 ReadiDrop Cell Viability Assays Flier, Rev B Click to download [ Add to Cart (Free) ]
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