2-D Electrophoresis Buffers and Reagents

The ReadyPrep 2-D starter kit is intended for first-time users of the PROTEAN IEF cell and ReadyStrip IPG strips. The kit contains tested premixed reagents and a reference manual with technical tips.

This E. coli lysate sample is performance-tested to generate a consistent pattern when used with ReadyPrep 2-D starter kit rehydration/sample buffer. Use as a control to validate your 2-D protocol.

Overlay agarose saves time in setting up second-dimension SDS-PAGE samples. Bromophenol blue tracking dye is incorporated into these solutions to allow monitoring of electrophoresis runs.

Detergents are used in electrophoresis when it is necessary to disrupt protein-lipid or protein-protein interactions. SDS, Tween 20, octylphenol ethoxylate and CHAPS solutions are available.

Reducing agents are commonly used during sample preparation to cleave disulfide bond crosslinks within proteins and between protein subunits. DTT, TBP and iodoacetamide are offered.

Bio-Lyte carrier ampholytes, supplied as clear aqueous solutions, are blended to give a complete range of isoelectric points for reproducible, linear pH gradients.