Propidium Iodide Solution

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Overview

Ready-to-use cell viability assay allows the exclusion of dead cells in flow cytometry experiments by intercalating between DNA and RNA base pairs.

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Description

Description

Commonly used in flow cytometry experiments for excluding dead cells, the propidium iodide solution enters a compromised cell and binds to double-stranded DNA/RNA by intercalating between base pairs.

Designed for ease of use in cell sorting and flow cytometry applications, the ReadiDrop™ propidium iodide cell viability assay removes the traditional manual weighing, pipetting, and dilution steps: simply add 1 or 2 drops of the propidium iodide solution to assess the health of your cells.

Key Benefits of the ReadiDrop Propidium Iodide Cell Viability Assay

  • Ideal for cell sorting — fluorescent molecules are suspended in PBS without preservatives
  • Stable at room temperature — keep a bottle of ReadiDrop propidium iodide cell viability assay by your cell sorter or cell analyzer for easy access
  • No more weighing, pipetting, or diluting — ready-to-use solution to gives 1 µg/ml as the final concentration

Features of the ReadiDrop Propidium Iodide Cell Viability Assay

  • Fluorophore Properties
    • Excitation maximum: 538 nm
    • Emission maximum: 617 nm
    • Optimal excitation laser: 561 nm or 488 nm
  • Fluorescent Spectrum

Fluorescent Spectrum

  • Molecular weight: 668.39 g/mol
  • Molecular formula: C27H32I2N4
  • Chemical structure

Chemical Structure

  • Specifications
    • Store at 15–30°C
    • Do not freeze

More Information

Learn more about the features of the S3e™ Cell Sorter and its associated consumables and accessories.

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1351101
9 ml, (3 x 3 ml) ready-to-use dropper bottles for dead cell exclusion

Documents

Documents

Number Description Options
6522
ReadiDrop Cell Viability Assays Flier, Rev B
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10049240
ReadiDrop™ Cell Viability Assays Product Insert, Rev A