Nuclear Staining Dyes

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PureBlu™ Nuclear Staining Dyes

Overview

Available Nuclear Staining Dyes
PureBlu™ Nuclear
Staining Dye
Excitation
Maximum, nm
Emission
Maximum, nm
Optimal Channel on
ZOE™ Cell Imager
Catalog
Number
DAPI 359 461 Blue 135-1303
Hoechst 33342 350 461 Blue 135-1304

 
PureBlu™ Nuclear Staining Dye One-Step Dilution

PureBlu Nuclear Staining Dye One-Step Dilution

Resuspend one tube of PureBlu Dye Powder in 500 µl of deionized H2O and dilute to 50 ml in PBS/media to prepare the working solution (PureBlu DAPI, 1 µg/ml (0.03 mM); PureBlu Hoechst 33342, 1.1 µg/ml (0.02 mM)).
 
Nuclear Staining Dyes Viewed with the ZOE™ Fluorescent Cell Imager

HeLa cells transfected with a GFP-expressing plasmid were counterstained with PureBlu™ DAPI or Hoechst 33342 Nuclear Staining Dyes and then imaged in a 6-well plate with the ZOE™ Fluorescent Cell Imager.

Nuclear Staining dyes

A, GFP-expressing HeLa cells; B, counterstained with PureBlu™ DAPI Nuclear Staining Dye; C, merged view: GFP + PureBlu DAPI staining.
 

Nuclear Staining dyes

A, GFP-expressing HeLa cells; B, counterstained with PureBlu™ Hoechst 33342 Nuclear Staining Dye; C, merged view: GFP + PureBlu Hoechst 33342 staining.


 

PureBlu™ DAPI and Hoechst 33342 Nuclear Staining Dyes are ready to use after a single dilution step — no weighing required.
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Ordering Information


Description

PureBlu™ Nuclear Staining Dyes are designed to specifically stain the nuclei of cells in fixed and unfixed samples for fluorescence microscopy and cell imaging applications.

Based on the well-recognized DAPI and Hoechst 33342 chemistries, PureBlu Dyes are offered in a ready-to-reconstitute, high-purity powder format. PureBlu DAPI and PureBlu Hoechst 33342 Dyes are both ready to use after a single dilution — no weighing required.

PureBlu Nuclear Staining Dye One-Step Dilution

Resuspend one tube of PureBlu Dye Powder in 500 µl of deionized H2O and dilute to
50 ml in PBS/media to prepare the working solution (PureBlu DAPI, 1 µg/ml (0.03 mM);
PureBlu Hoechst 33342, 1.1 µg/ml (0.02 mM)).
 

Features and Benefits of PureBlu Nuclear Staining Dyes

  • High purity formulation for quality results in challenging experiments
  • Pre-aliquoted powder format eliminates time-consuming weighing steps
  • Convenient format allows you to generate working solution with only one dilution step after resuspension
  • Compatible with multicolor experiments

Applications and Uses of PureBlu Nuclear Staining Dyes

  • Nuclear staining
  • Microscopy and cell imaging
  • Multicolor experiments

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PureBlu™ DAPI Nuclear Staining Dye

1351303
Pkg of 5 vials, 50 µg each, DAPI nuclear staining dye powder

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$123.00 
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PureBlu™ Hoechst 33342 Nuclear Staining Dye

1351304
Pkg of 5 vials, 56 µg each, Hoechst 33342 nuclear staining dye powder

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$123.00 
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Documents

Number Description Options
10043282 PureBlu™ DAPI Nuclear Staining Dye Product Insert, Rev A Click to download
10043283 PureBlu™ Hoechst 33342 Nuclear Staining Dye Product Insert, Rev A Click to download
6607 PureBlu™ Nuclear Staining Dyes Flier, Ver B Click to download [ Add to Cart (Free) ]

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